Fabrication of chitosan/hydroxylapatite composite rods with a layer-by-layer structure for fracture fixation
| Autor: | Ximing Pu, Qiqing Zhang, Zhen-zhen Sun, Zhen-Qing Hou, Qingqing Yao, Yun Yang |
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| Rok vydání: | 2011 |
| Předmět: |
Chitosan
Fabrication Materials science business.industry Layer by layer Composite number Biomedical Engineering Structural engineering Hydroxylapatite Rod Cell Line Biomaterials chemistry.chemical_compound Fractures Bone Mice Durapatite chemistry Fracture fixation Bone Substitutes Materials Testing Animals Nanoparticles Christian ministry Composite material business |
| Zdroj: | Journal of biomedical materials research. Part B, Applied biomaterials. 100(5) |
| ISSN: | 1552-4981 |
| Popis: | A composite rod for fracture fixation using chitosan (CHI)/hydroxylapatite (HA) was prepared by means of in situ precipitation, which had a layer-by-layer structure, good mechanical properties, and cell compatibilities. The CHI/HA composite rods were precipitated from the chitosan solution with calcium and phosphorus precursors, followed by treatment with a tripolyphosphate-trisodium phosphate solution (pH13) to crosslink the CHI and to hydrolyze the calcium phosphates to nanocrystalline HA. The results of FTIR, XRD, and TEM measurements confirmed that HA had been formed within the CHI matrix. The effects of the CHI/HA ratios (20/0, 20/1, 20/2, 20/4, and 20/5, w/w) on the mechanical properties were investigated. At the CHI/HA ratio of 20/4 (w/w), the bending strength and modulus of the rods were 133 MPa and 6.8 GPa, respectively. Pre-osteoblast MC3T3-E1 cells were cultured in an extract of the CHI/HA rods (20/4, w/w) to study the cell compatibilities of the composite. The observations indicated that the CHI/HA composite could promote the growth of MC3T3-E1 cells better than the composite without HA (p0.05). Furthermore, the co-cultivation of the cells and the CHI/HA composite showed that cells fully spread on the surface of the composite with an obvious cytoskeleton organization, which also revealed that the CHI/HA composite had a good biocompatibility. |
| Databáze: | OpenAIRE |
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