H 2 O 2 Production in Species of the Lactobacillus acidophilus Group: a Central Role for a Novel NADH-Dependent Flavin Reductase
| Autor: | Jos C. Arents, Rosanne Y. Hertzberger, M. Joost Teixeira de Mattos, R. David Pridmore, Christof Gysler, Henk L. Dekker, Michiel Kleerebezem |
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| Přispěvatelé: | Molecular Microbial Physiology (SILS, FNWI), Mass Spectrometry of Biomacromolecules (SILS, FNWI) |
| Rok vydání: | 2014 |
| Předmět: |
johnsonii ncc-533
FMN Reductase Physiology Coenzymes Flavoprotein Flavin mononucleotide Flavin group Reductase Applied Microbiology and Biotechnology chemistry.chemical_compound FMN reductase Flavin reductase hydrogen-peroxide production Host-Microbe Interactomics activated-receptor-gamma Lactobacillus johnsonii lactic-acid bacteria Flavin adenine dinucleotide Ecology biology Genetic Complementation Test crystal-structure food and beverages Hydrogen Peroxide NAD biology.organism_classification Molecular biology alkyl hydroperoxide reductase streptococcus-pneumoniae Molecular Weight Kinetics Lactobacillus chemistry Biochemistry escherichia-coli biology.protein amphibacillus-xylanus Gene Deletion pseudomonas-putida Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology, 80(7), 2229-2239. American Society for Microbiology Applied and Environmental Microbiology, 80(7), 2229-2239 Appl. Environ. Microbiol. April 2014 vol. 80 no. 7 2229-2239 Applied and Environmental Microbiology 80 (2014) 7 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.04272-13 |
| Popis: | Hydrogen peroxide production is a well-known trait of many bacterial species associated with the human body. In the presence of oxygen, the probiotic lactic acid bacterium Lactobacillus johnsonii NCC 533 excretes up to 1 mM H 2 O 2 , inducing growth stagnation and cell death. Disruption of genes commonly assumed to be involved in H 2 O 2 production (e.g., pyruvate oxidase, NADH oxidase, and lactate oxidase) did not affect this. Here we describe the purification of a novel NADH-dependent flavin reductase encoded by two highly similar genes ( LJ _ 0548 and LJ _ 0549 ) that are conserved in lactobacilli belonging to the Lactobacillus acidophilus group. The genes are predicted to encode two 20-kDa proteins containing flavin mononucleotide (FMN) reductase conserved domains. Reductase activity requires FMN, flavin adenine dinucleotide (FAD), or riboflavin and is specific for NADH and not NADPH. The K m for FMN is 30 ± 8 μM, in accordance with its proposed in vivo role in H 2 O 2 production. Deletion of the encoding genes in L. johnsonii led to a 40-fold reduction of hydrogen peroxide formation. H 2 O 2 production in this mutant could only be restored by in trans complementation of both genes. Our work identifies a novel, conserved NADH-dependent flavin reductase that is prominently involved in H 2 O 2 production in L. johnsonii . |
| Databáze: | OpenAIRE |
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