A vaccine targeting basic fibroblast growth factor elicits a protective immune response against murine melanoma
| Autor: | Neng-Lian Li, Lu-Lu Luo, Yun-Yan Duan, Xiao-Ping Zhang, Zhen-Dong Li, Ying-Dong Li, Yong-Qi Liu, Li-Ping Yang, Hai-Xia Song, Xiao-Rong Xie, Fang-Yu An, Chao Guo |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
CD31 Cancer Research Angiogenesis medicine.medical_treatment Basic fibroblast growth factor Biology Cancer Vaccines 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Immune system medicine Cytotoxic T cell Animals Humans Melanoma Pharmacology Immunotherapy medicine.disease Disease Models Animal 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Cancer research Molecular Medicine Female Fibroblast Growth Factor 2 Adjuvant Research Paper |
| Popis: | Tumor growth and metastasis are closely related to angiogenesis. Basic fibroblast growth factor(bFGF) is an angiogenic factor, and up-regulated expression of bFGF plays a crucial role in the development and metastasis of melanoma. Therefore, in this study, we sought to achieve antitumor activity by immunity targeting bFGF which would inhibit tumor angiogenesis and simultaneously induce bFGF specific cytotoxic T lymphocytes to kill melanoma cells. A human bFGF protein was used as exogenous antigen, coupled with a saponin-liposome adjuvant formulation to enhance CTL response. The results showed that the immunity induced strong immune response and produced prominent anti-cancer activities. CD31 immunohistochemistry and alginate-encapsulated tumor cell assay displayed that tumor angiogenesis was effectively inhibited. Further, the higher production of IFN-γ and cytotoxic T lymphocyte killing assay suggested that the anti-cancer activities may mainly depend on cellular immune response, which could cause the inhibition of tumor angiogenesis and specific killing of tumor cells by bFGF-specific cytotoxic T lymphocytes. We concluded that immunotherapy targeting bFGF may be a prominent strategy for melanoma, and that the adjuvant formulation of saponin-liposome is very desirable in enhancing cytotoxic T lymphocytes response. |
| Databáze: | OpenAIRE |
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