Type I Diabetes Mellitus and Insulin Therapy on Bone Microarchitecture, Composition and Mechanical Properties
| Autor: | Carlos José Soares, Pedro Henrique Justino Oliveira Limirio, Priscilla Barbosa Ferreira Soares, Paula Dechichi, Jessyca Figueira Venâncio, Gustavo Davi Rabelo |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Endocrinology Internal medicine Diabetes mellitus medicine Cortical Bone Animals Humans Insulin Minerals business.industry Type i diabetes mellitus X-Ray Microtomography Streptozotocin medicine.disease Rats medicine.anatomical_structure Diabetes Mellitus Type 1 Composition (visual arts) Cortical bone business Bone surface medicine.drug Tissue volume |
| Zdroj: | Current diabetes reviews. 18(8) |
| ISSN: | 1875-6417 |
| Popis: | Background: The aim of this study was to evaluate the microarchitecture, composition and mechanical properties of cortical bone of rats with type I diabetes mellitus (TIDM) and submitted to insulin therapy (IT). Methods: Thirty rats were divided into three groups (n=10): non-diabetic, diabetic and diabetic+insulin. TIDM was induced by intravenous injection of streptozotocin. In diabetic+insulin group, 4IU insulin was administered twice per day (1I U at 7 am and 3I U at 7 pm). The animals were euthanized five weeks after TIDM induction; the tibiae were removed and submitted to microcomputed tomography (micro-CT, 8 μm), fourier transform infrared spectroscopy (FTIR) and dynamic microhardness indentation. Results: Micro-CT analysis showed that diabetic group had lower bone surface/tissue volume ratio (BS/BV) (p=0.018), cortical thickness (Ct.Th) (p Conclusion: TIDM negatively affects bone microarchitecture, collagen maturation, mineralization and bone microhardness. Moreover, insulin minimized the effect of TIDM on cortical thickness and organic/mineral matrix. |
| Databáze: | OpenAIRE |
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