Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis

Autor: T. P. Rocha, Andre L. Moreira, Fernando Augusto Soares, Andrew G. Nicholson, Vera Luiza Capelozzi, V. K. de Sá, Lina Carvalho, T Y Takagaki
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Life Sciences & Biomedicine - Other Topics
Male
Pathology
Medicine (General)
Lung Neoplasms
Physiology
Kaplan-Meier Estimate
Research & Experimental Medicine
Biochemistry
Severity of Illness Index
ANGIOGENESIS
BRONCHIAL EPITHELIUM
Metaplasia
General Pharmacology
Toxicology and Pharmaceutics
Biology (General)
Pre-neoplastic lung/bronchial lesions
Glucuronosyltransferase
lcsh:QH301-705.5
Aged
80 and over
lcsh:R5-920
General Neuroscience
Bronchial Neoplasms
Hyaluronidases and hyaluronan synthases
CD44 RECEPTOR
LOCALIZATION
11 Medical And Health Sciences
General Medicine
Hyperplasia
respiratory system
Middle Aged
Prognosis
TUMORS
Immunohistochemistry
Squamous metaplasia
PROSTATE-CANCER
Neoplasm Proteins
Medicine
Research & Experimental
Carcinoma
Squamous Cell
GROWTH
Adenocarcinoma
Female
SQUAMOUS-CELL CARCINOMA
medicine.symptom
Lung cancer
lcsh:Medicine (General)
Life Sciences & Biomedicine
Adult
medicine.medical_specialty
QH301-705.5
Immunology
Biophysics
Hyaluronoglucosaminidase
Ocean Engineering
Basal Cell Hyperplasia
Statistics
Nonparametric
LUNG-CANCER
R5-920
medicine
Humans
Atypical adenomatous hyperplasia
Clinical Investigation
Biology
Moderate Dysplasia
Aged
Science & Technology
Neurology & Neurosurgery
business.industry
Morphometry
Cell Biology
medicine.disease
CANCER STATISTICS
lcsh:Biology (General)
Multivariate Analysis
business
Cell Adhesion Molecules
Hyaluronan Synthases
Precancerous Conditions
Zdroj: Brazilian Journal of Medical and Biological Research
Brazilian Journal of Medical and Biological Research, Vol 48, Iss 11, Pp 1039-1047 (2015)
Brazilian Journal of Medical and Biological Research v.48 n.11 2015
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Volume: 48, Issue: 11, Pages: 1039-1047, Published: 28 AUG 2015
ISSN: 1414-431X
0100-879X
Popis: We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, adenomatous hyperplasia, severe dysplasia, squamous cell carcinoma and adenocarcinoma. Matched lung parenchyma tissue samples included 25 squamous cell carcinomas and 31 adenocarcinomas. Immunohistochemistry was performed to analyze for the distribution of hyaluronidase (Hyal)-1 and −3, and hyaluronan synthases (HAS)-1, −2, and −3. Hyal-1 showed significantly higher expression in basal cell hyperplasia than in moderate dysplasia (P=0.01), atypical adenomatous hyperplasia (P=0.0001), or severe dysplasia (P=0.03). Lower expression of Hyal-3 was found in atypical adenomatous hyperplasia than in basal cell hyperplasia (P=0.01) or moderate dysplasia (P=0.02). HAS-2 was significantly higher in severe dysplasia (P=0.002) and in squamous metaplasia (P=0.04) compared with basal cell hyperplasia. HAS-3 was significantly expressed in basal cell hyperplasia compared with atypical adenomatous hyperplasia (P=0.05) and severe dysplasia (P=0.02). Lower expression of HAS-3 was found in severe dysplasia compared with squamous metaplasia (P=0.01) and moderate dysplasia (P=0.01). Epithelial Hyal-1 and −3 and HAS-1, −2, and −3 expressions were significantly higher in pre-neoplastic lesions than in neoplastic lesions. Comparative Cox multivariate analysis controlled by N stage and histologic tumor type showed that patients with high HAS-3 expression in pre-neoplastic cells obtained by lung/bronchial biopsy presented a significantly higher risk of death (HR=1.19; P=0.04). We concluded that localization of Hyal and HAS in lung/bronchial pre-neoplastic and neoplastic lesions was inversely related to malignancy, which implied that visualizing these factors could be a useful diagnostic procedure for suspected lung cancer. Finalizing this conclusion will require a wider study in a randomized and prospective trial.
Databáze: OpenAIRE
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