Polarization of the yeast pheromone receptor requires its internalization but not actin-dependent secretion
| Autor: | Madhushalini E Sukumar, Robert A. Arkowitz, Reagan DeFlorio, Dmitry Suchkov, David E. Stone, Amber Ismael, Edward Draper |
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| Přispěvatelé: | Institute of Developmental Biology and Cancer (IBDC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA) |
| Rok vydání: | 2010 |
| Předmět: |
MESH: Signal Transduction
MESH: Chemotaxis MESH: Receptors Pheromone Pheromones 0302 clinical medicine Yeasts Internalization Receptor [SDV.BDD]Life Sciences [q-bio]/Development Biology media_common MESH: Pheromones 0303 health sciences Mating projection MESH: Yeasts Chemotaxis Eukaryota Articles MESH: Saccharomyces cerevisiae Receptors Pheromone Transport protein Cell biology MESH: Eukaryota Protein Transport Signal transduction Protein Binding Signal Transduction MESH: Protein Transport MESH: GTP-Binding Proteins MESH: Mutation G protein media_common.quotation_subject B-cell receptor education macromolecular substances Saccharomyces cerevisiae Biology MESH: Actins 03 medical and health sciences Neurotransmitter receptor GTP-Binding Proteins MESH: Protein Binding Molecular Biology 030304 developmental biology Cell Membrane Cell Biology Actins Membrane Trafficking Mutation 030217 neurology & neurosurgery MESH: Cell Membrane |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2010, 21 (10), pp.1737-52. ⟨10.1091/mbc.E09-08-0706⟩ |
| ISSN: | 1939-4586 |
| DOI: | 10.1091/mbc.E09-08-0706⟩ |
| Popis: | The data presented in this paper suggest that pheromone-induced receptor phosphorylation and internalization, but not actin-dependent directed secretion, are required to establish receptor polarity. In the best understood models of eukaryotic directional sensing, chemotactic cells maintain a uniform distribution of surface receptors even when responding to chemical gradients. The yeast pheromone receptor is also uniformly distributed on the plasma membrane of vegetative cells, but pheromone induces its polarization into “crescents” that cap the future mating projection. Here, we find that in pheromone-treated cells, receptor crescents are visible before detectable polarization of actin cables and that the receptor can polarize in the absence of actin-dependent directed secretion. Receptor internalization, in contrast, seems to be essential for the generation of receptor polarity, and mutations that deregulate this process confer dramatic defects in directional sensing. We also show that pheromone induces the internalization and subsequent polarization of the mating-specific Gα and Gβ proteins and that the changes in G protein localization depend on receptor internalization and receptor–Gα coupling. Our data suggest that the polarization of the receptor and its G protein precedes actin polarization and is important for gradient sensing. We propose that the establishment of receptor/G protein polarity depends on a novel mechanism involving differential internalization and that this serves to amplify the shallow gradient of activated receptor across the cell. |
| Databáze: | OpenAIRE |
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