The Polymerized Bovine Hemoglobin-Based Oxygen-Carrying Solution (HBOC-201) Is Not Toxic to Neural Cells in Culture
| Autor: | John D. Josephs, Michael R. Davis, Jeffrey D. Kerby, Delio P. Ortegon, Donald H. Jenkins, David L. Smith, Patricia S. Dixon, Deborah L Mueller |
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| Rok vydání: | 2002 |
| Předmět: |
Lysis
Critical Care and Intensive Care Medicine Sensitivity and Specificity Hemopure Andrology Hemoglobins Blood Substitutes medicine Animals Cells Cultured Probability Neurons Analysis of Variance Fetus Cell growth business.industry Neurotoxicity medicine.disease Rats Cell culture Toxicity Immunology Cattle Surgery Hemoglobin business Cell Division |
| Zdroj: | The Journal of Trauma: Injury, Infection, and Critical Care. 53:1068-1072 |
| ISSN: | 0022-5282 |
| Popis: | Background: Recent data suggest that a neurotoxic effect of blood or its components may contribute to secondary neural cell dysfunction. This study investigated the effects of HBOC-201 (Hemopure) and purified human hemoglobin (hHgb) on rat fetal neural cell culture. Methods: Neural cell cultures were exposed to HBOC-201 and hHgb (0.02, 0.2, 2.0, and 6.5 g/dL) for 24 hours, and then analyzed for proliferation, metabolism, and neurolysis. Results: Cultures exposed to HBOC-201 maintained levels of proliferation and metabolism similar to controls while demonstrating no cellular lysis. However, cultures exposed to hHgb demonstrated decreased proliferation after exposure to 0.2, 2.0, and 6.5 g/dL hHgb (14,252.14, 3,221.89, and 343.12 vs. 19,509.53; p < 0.05) when compared with controls. In addition, cultures exposed to hHgb demonstrated decreased metabolic activity and increased cell lysis when compared with controls (p < 0.05). Conclusion: Cultures exposed to HBOC-201 displayed sustained metabolic activity and proliferation, and demonstrated no neurolysis, suggesting that HBOC-201 does not display the toxic characteristics of hHgb. |
| Databáze: | OpenAIRE |
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