Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs
| Autor: | Sabrina de Souza Ferreira, Suelen Silvana dos Santos, Lavínia Maria Dal'Mas Romera, Sandro Rogério de Almeida, Fernando Henrique Galvão Tessaro, Fernanda Peixoto Barbosa Nunes, Paula Regina de Souza, Joilson O. Martins, Felipe Beccaria Casagrande |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Article Subject Endocrinology Diabetes and Metabolism medicine.medical_treatment Spleen Inflammation lcsh:Diseases of the endocrine glands. Clinical endocrinology Diabetes Mellitus Experimental 03 medical and health sciences Mice Endocrinology Diabetes mellitus PULMÃO Medicine Animals Hypoglycemic Agents Insulin Lung Paracoccidioides brasiliensis B-Lymphocytes lcsh:RC648-665 biology medicine.diagnostic_test business.industry Paracoccidioidomycosis Interleukin Paracoccidioides Dendritic Cells biology.organism_classification medicine.disease Killer Cells Natural Mice Inbred C57BL 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure Immunology medicine.symptom business Research Article |
| Zdroj: | Journal of Diabetes Research Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Journal of Diabetes Research, Vol 2018 (2018) |
| ISSN: | 2314-6745 |
| DOI: | 10.1155/2018/6209694 |
| Popis: | Paracoccidioidomycosis, a key issue for Brazilian health service, can be aggravated in patients with impaired immunological responses, such as diabetic patients. We evaluated the role of insulin in inflammatory parameters in diabetic and nondiabetic mice using a systemic mycosis Paracoccidioides brasiliensis (Pb) model. Diabetic C57BL-6 mice and controls were infected with Pb18 and treated with insulin for 12 days prior to experiments. After 55 days, infected diabetic mice exhibited fewer leukocytes in both peritoneal lavage fluid (PeLF) and bronchoalveolar lavage fluid and reduced secretion of interleukin- (IL-) 6 in lungs. In addition, diabetic mice presented a reduced influx of TCD4+ cells, TCD8+ cells, B lymphocytes, NK cells, and dendritic cells compared to control infected groups. Insulin treatment restored the leukocyte number in PeLF and restored the presence of B lymphocytes, dendritic cells, and NK cells in lungs of diabetic animals. The data suggest that diabetic mice present impaired immunological response to Pb18 infection and insulin modulates inflammation by reducing IL-6 levels in lung and CINC-1 levels in spleen and liver homogenates, restoring leukocyte concentrations in PeLF and also restoring populations of dendritic cells and B lymphocytes in lungs of diabetic mice, permitting the host to better control the infection. |
| Databáze: | OpenAIRE |
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