Towards gas chromatography–mass spectrometry coupling protocols for both identifying and quantification essential oils of Thymus capitatus Hoff et Link
Autor: | Manef Abderrabba, Henri Planche, Lassaad Hedhili, Mehrez Romdhane |
---|---|
Rok vydání: | 2005 |
Předmět: |
Chromatography
Chemistry Organic Chemistry Analytical chemistry Reproducibility of Results General Medicine Mass spectrometry Biochemistry Gas Chromatography-Mass Spectrometry food.food Analytical Chemistry law.invention Thymus Plant Mass food law Calibration Oils Volatile Flame ionization detector Thymus capitatus Gas chromatography Ion trap Gas chromatography–mass spectrometry Essential oil |
Zdroj: | Journal of Chromatography A. 1064:129-134 |
ISSN: | 0021-9673 |
Popis: | Essential oil of Thymus capitatus Hoff et Link is analysed by using four techniques: GC/pyrolyse/MS, GC/FID, electronic impact GC/MS (quadripole), and GC/MS (ion trap). Both major and trace components are analysed. The GC/pyrolyse/MS coupling provides reference to the exact mass compositions without any need of the previously purified references, neither for major or trace components. The comparison between this reference analysis and GC/FID shows that the FID response coefficients may vary by a mean 7% from one component to another. As it was expected, quadripole or ion trap response coefficients vary to a much greater extent (a mean 25%), although the two MS techniques response coefficients are first order consistent. We conclude that GC/MS coupling could be used not only as it is usual for reliable identifications, but also for a complete quantitative routine analysis of essential oils. Expected precision could be very similar to GC/FID precision provided correcting species by species the MS analysis by a mean value of the response coefficient measured for the MS 70 eV electronic impact ionisation technologies. The GC/pyrolyse/MS coupling is proposed as a relevant tool for analysing reference samples containing trace natural species that could not be purified. |
Databáze: | OpenAIRE |
Externí odkaz: |