Orcokinins contribute to the regulation of vitellogenin transcription in the cockroach Blattella germanica
| Autor: | Xavier Bellés, Sheila Ons, José L. Maestro |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, European Commission, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Physiology Otras Ciencias Biológicas Circadian clock Fat Body Molecular Sequence Data Brain-gut peptides Ciencias Biológicas Vitellogenin Fat body Vitellogenins biology.animal Internal medicine Insect reproduction medicine Gene silencing Animals Amino Acid Sequence Regulation of gene expression Brain Chemistry Cockroach Orcokinin biology Vitellogenesis Neuropeptides Blattellidae Cell biology Gastrointestinal Tract Juvenile Hormones Endocrinology Gene Expression Regulation Insect Science Juvenile hormone biology.protein Female RNA Interference CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1879-1611 |
| Popis: | Orcokinins (OKs) are neuropeptides that were first identified in crustacean through their myotropic activity. In insects, the OK gene gives rise to two mRNAs coding for two different families of conserved mature neuropeptides: OKA and OKB. Although OKs are conserved in many insect species, its physiological role in this animal class is not fully understood. Until now prothoracicotropic, regulatory of light entrainment to the circadian clock and >awakening> activities have been reported for these peptides in different insect species. Here we report the identification of OKA and OKB precursors in the cockroach Blattella germanica. OKA mRNA was detected in brain, whereas OKB mRNA was detected both in brain and midgut. In vivo silencing of OK precursors suggests the involvement of OK gene products in the regulation of vitellogenin expression in the fat body, an action that appears to be independent of juvenile hormone. This is the first time that a function of this kind has been reported for OKs. This work was supported by the Spanish MICINN (Grants CGL2008-03517/BOS to XB and BFU2010-15906 to JLM) and MINECO (Grant CGL2012-36251 to XB), and Catalan Government (2014 SGR 619). The research has also benefited from FEDER funds. SO was recipient of an external fellowship from National Council of Scientific and Technical Research (CONICET-Argentina) to work in the Institute of Evolutionary Biology, in Barcelona. |
| Databáze: | OpenAIRE |
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