Surface display on staphylococci: a comparative study
| Autor: | Hans Binz, Stefan Ståhl, Patrik Samuelson, Alain Robert, Mathias Uhlén, Christine Andreoni, Thien Ngoc Nguyen, Thomas Bächi |
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| Rok vydání: | 1996 |
| Předmět: |
Recombinant Fusion Proteins
Staphylococcus Genetic Vectors Biophysics Heterologous Staphylococcal protein A Respiratory syncytial virus Biochemistry Fluorescence Microbiology Flow cytometry Viral Proteins Transformation Genetic Viral Envelope Proteins Structural Biology Genetics medicine Humans Staphylococcus carnosus Receptor Molecular Biology chemistry.chemical_classification HN Protein biology medicine.diagnostic_test Staphylococcus xylosus Cell Biology Cell sorting Surface display Flow Cytometry biology.organism_classification Immunohistochemistry Respiratory Syncytial Viruses Bacterial vaccine Microscopy Electron chemistry Fluorescence-activated cell sorting Glycoprotein |
| Zdroj: | FEBS Letters. 390:327-333 |
| ISSN: | 0014-5793 |
| Popis: | Two different host-vector expression systems, designed for cell surface display of heterologous receptors on Staphylococcus xylosus and Staphylococcus carnosus, respectively, were compared for the surface display of four variants of a 101 amino acid region derived from the G glycoprotein of human respiratory syncytial virus (RSV). Surface localization of the different chimeric receptors was evaluated by a colorimetric assay and by fluorescence-activated cell sorting. It was concluded that the S. carnosus system was better both in the ability to translocate inefficiently secreted peptides and in the number of exposed hybrid receptors. The potential use of the described staphylococci as live bacterial vaccine vehicles or alternatives to filamentous phages for surface display of protein libraries is discussed. |
| Databáze: | OpenAIRE |
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