Visualization of antennal lobe glomeruli activated by nonappetitive D-limonene and appetitive 1-octen-3-ol odors via two types of olfactory organs in the blowfly Phormia regina

Autor: Tomoyosi Nisimura, Shunnya Habe, Toru Maeda, Mamiko Ozaki, Tatsuya Uebi
Rok vydání: 2020
Předmět:
Zdroj: Zoological Letters, Vol 6, Iss 1, Pp 1-13 (2020)
Zoological Letters
ISSN: 2056-306X
DOI: 10.1186/s40851-020-00167-3
Popis: Appetite or feeding motivation relies significantly on food odors. In the blowflyPhormia regina, feeding motivation for sucrose is decreased by the odor ofd-limonene but increased by the odor of 1-octen-3-ol odor. These flies have antennal lobes (ALs) consisting of several tens of glomerular pairs as a primary olfactory center in the brain. Odor information from different olfactory organs—specifically, the antennae and maxillary palps—goes to the corresponding glomeruli. To investigate how odors differently affect feeding motivation, we identified the olfactory organs and glomeruli that are activated by nonappetitive and appetitive odors. We first constructed a glomerular map of the antennal lobe inP. regina. Anterograde fluorescence labeling of antennal and maxillary afferent nerves, both of which project into the contralateral and ipsilateral ALs, revealed differential staining in glomerular regions. Some of the axonal fiber bundles from the antennae and maxillary palps projected to the subesophageal ganglion (SOG). We visualized the activation of the glomeruli in response to odor stimuli by immunostaining phosphorylated extracellular signal-regulated kinase (pERK). We observed different glomerulus activation under different odor stimulations. Referring to our glomerular map, we determined that antennal exposure tod-limonene odor activated the DA13 glomeruli, while exposure of the maxillary palps to 1-octen-3-ol activated the MxB1 glomeruli. Our results indicated that a nonappetitive odor input from the antennae and an appetitive odor input from the maxillary palps activate different glomeruli in the different regions of ALs in the blowflyP. regina. Collectively, our findings suggest that compartmentalization of glomeruli in AL is essential for proper transmission of odor information.
Databáze: OpenAIRE
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