γ-TuRC Heterogeneity Revealed by Analysis of Mozart1
| Autor: | Tovey, C, Tubman, C, Hamrud, E, Zhu, Z, Dyas, A, Butterfield, A, Fyfe, A, Johnson, E, Conduit, P |
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| Přispěvatelé: | Conduit, Paul [0000-0002-7822-1191], Apollo - University of Cambridge Repository |
| Rok vydání: | 2018 |
| Předmět: |
Male
centrosomin microtubule organizing center Gene Expression Profiling Cell Cycle Proteins Spermatozoa Article spermatogenesis Basal Bodies Mitochondria centriole adjunct MTOC centrosome Drosophila melanogaster Animals Drosophila Proteins Amino Acid Sequence gamma-tubulin ring complex Mozart1 g-TuRC Microtubule-Associated Proteins Sequence Alignment microtubule |
| Zdroj: | Current Biology |
| Popis: | Summary Microtubules are essential for various cell processes [1] and are nucleated by multi-protein γ-tubulin ring complexes (γ-TuRCs) at various microtubule organizing centers (MTOCs), including centrosomes [2, 3, 4, 5, 6]. Recruitment of γ-TuRCs to different MTOCs at different times influences microtubule array formation, but how this is regulated remains an open question. It also remains unclear whether all γ-TuRCs within the same organism have the same composition and how any potential heterogeneity might influence γ-TuRC recruitment. MOZART1 (Mzt1) was recently identified as a γ-TuRC component [7, 8] and is conserved in nearly all eukaryotes [6, 9]. Mzt1 has so far been studied in cultured human cells, yeast, and plants; its absence leads to failures in γ-TuRC recruitment and cell division, resulting in cell death [7, 9, 10, 11, 12, 13, 14, 15]. Mzt1 is small (∼8.5 kDa), binds directly to core γ-TuRC components [9, 10, 14, 15], and appears to mediate the interaction between γ-TuRCs and proteins that tether γ-TuRCs to MTOCs [9, 15]. Here, we use Drosophila to investigate the function of Mzt1 in a multicellular animal for the first time. Surprisingly, we find that Drosophila Mzt1 is expressed only in the testes and is present in γ-TuRCs recruited to basal bodies, but not to mitochondria, in developing sperm cells. mzt1 mutants are viable but have defects in basal body positioning and γ-TuRC recruitment to centriole adjuncts; sperm formation is affected and mutants display a rapid age-dependent decline in sperm motility and male fertility. Our results reveal that tissue-specific and MTOC-specific γ-TuRC heterogeneity exist in Drosophila and highlight the complexity of γ-TuRC recruitment in a multicellular animal. Graphical Abstract Highlights • The protein composition of gamma-tubulin ring complexes (γ-TuRCs) varies in flies • Drosophila Mozart1 is non-essential, and its expression is restricted to the testes • Mozart1 is required for γ-TuRC recruitment specifically to basal bodies • mzt1 mutants have defects in basal body positioning and sperm formation Tovey et al. show that γ-TuRCs do not all have the same protein composition in Drosophila. This heterogeneity is determined by the γ-TuRC protein Mzt1, which is expressed only in fly testes. Mzt1 is surprisingly not required for cell division but is instead required for the recruitment of γ-TuRCs to basal bodies and for correct sperm formation. |
| Databáze: | OpenAIRE |
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