Thein silicoidentification and characterization of a bread wheat/Triticum militinaeintrogression line

Autor: Andrzej Kilian, Eva Komínková, Irena Jakobson, Hana Šimková, Miroslav Valárik, Mihaela Martis, Jan Vrána, Ljudmilla Timofejeva, Kadri Järve, Barbora Balcárková, Michael Abrouk, Jaroslav Doležel, Elodie Rey
Rok vydání: 2016
Předmět:
Genetic Markers
0106 biological sciences
0301 basic medicine
comparative analysis
linkage drag
DNA
Plant
Introgression
Plant Science
Chromosomal rearrangement
Plant disease resistance
Biology
Genes
Plant
01 natural sciences
Genome
GenomeZipper
Chromosomes
Plant
Translocation
Genetic
03 medical and health sciences
Ascomycota
chromosome rearrangement
Computer Simulation
Gene
Research Articles
Triticum
Disease Resistance
Plant Diseases
Genetics
Base Sequence
Chromosome Mapping
food and beverages
Chromosome
Bread
Alien Introgression
Chromosome Rearrangement
Chromosome Translocation
Comparative Analysis
Genomezipper
Linkage Drag
030104 developmental biology
alien introgression
Genetic marker
Chromosome Arm
Agronomy and Crop Science
chromosome translocation
Research Article
Microsatellite Repeats
010606 plant biology & botany
Biotechnology
Zdroj: Plant Biotechnol. J. 15, 249-256 (2017)
Plant Biotechnology Journal
ISSN: 1467-7644
DOI: 10.1111/pbi.12610
Popis: The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tähti × T. militinae (Tm) is known to be due to the incorporation of a Tm segment into the long arm of chromosome 4A. Here, a newly developed in silico method termed rearrangement identification and characterization (RICh) has been applied to characterize the introgression. A virtual gene order, assembled using the GenomeZipper approach, was obtained for the native copy of chromosome 4A; it incorporated 570 4A DArTseq markers to produce a zipper comprising 2132 loci. A comparison between the native and introgressed forms of the 4AL chromosome arm showed that the introgressed region is located at the distal part of the arm. The Tm segment, derived from chromosome 7G, harbours 131 homoeologs of the 357 genes present on the corresponding region of Chinese Spring 4AL. The estimated number of Tm genes transferred along with the disease resistance gene was 169. Characterizing the introgression's position, gene content and internal gene order should not only facilitate gene isolation, but may also be informative with respect to chromatin structure and behaviour studies.
Databáze: OpenAIRE
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