Vascular effects of H2S-donors: Fluorimetric detection of H2S generation and ion channel activation in human aortic smooth muscle cells
| Autor: | Vincenzo Calderone, Alma Martelli, Valentina Citi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Membrane potential
0303 health sciences Vascular smooth muscle Chemistry Hydrogen sulfide Potassium chemistry.chemical_element DiBac4(3) Human aortic smooth muscle cells (HASMCs) Hydrogen sulfide detection Hyperpolarization (biology) equipment and supplies Potassium channel Potassium channels WSP-1 03 medical and health sciences chemistry.chemical_compound Electrophysiology 0302 clinical medicine 030220 oncology & carcinogenesis Hyperpolarizing effect Biophysics Ion channel 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493995271 |
| Popis: | Hydrogen sulfide (H2S) evokes vascular effects through several mechanisms including in wide part the activation of some ion channels such as ATP-sensitive potassium (KATP) channels and voltage-gated Kv7 potassium channels. Electrophysiological methods are very accurate, but they require high expertise and high specialized equipment. A more manageable fluorimetric technique which allows to record the membrane potential variations by the employment of an anionic bis-oxonol dye named DiBac4(3) with the administration of different blockers of several potassium channels could be useful to discover the targets of H2S-induced vascular hyperpolarization. Coupled with this technique, a fluorimetric detection (by the use of WSP-1 dye) of H2S generation in human vascular smooth muscle cells after H2S-donor administration could confirm the ability of these molecules to evoke the hyperpolarizing effect through the H2S release. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|