High genetic diversity and differentiation of the Babesia ovis population in Turkey

Autor: Munir Aktas, Serkan Bakirci, Tülin Karagenç, Anabela Mira, Varda Shkap, William Weir, Leonhard Schnittger, Selin Hacilarlioglu, Monica Florin-Christensen, Ahmet Hakan Ünlü, Brian Shiels, Hüseyin Bilgin Bilgiç, Tamara Carletti
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Turquía
Genotype
Turkey
040301 veterinary sciences
Population
Babesia ovis
Babesia
Sheep Diseases
Zoology
Satellite DNA
Biology
Tick
Polymerase Chain Reaction
Ovinos
0403 veterinary science
03 medical and health sciences
Tipificación de Secuencias Multilocus
Estructura de la Población
Babesiosis
medicine
Animals
Parasite hosting
education
ADN Satélite
Population Structure
030304 developmental biology
Caprinos
0303 health sciences
Genetic diversity
education.field_of_study
Sheep
General Veterinary
General Immunology and Microbiology
Genetic diversity (as resource)
Goats
Genetic Variation
04 agricultural and veterinary sciences
General Medicine
DNA
Protozoan
biology.organism_classification
medicine.disease
Tick Infestations
Rhipicephalus
Multilocus sequence typing
Diversidad genética (como recurso)
Microsatellite Repeats
Multilocus Sequence Typing
Zdroj: Transboundary and Emerging Diseases 67 (Supl. 2) : 26-35 (Julio 2020)
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
instacron:INTA
ISSN: 1865-1674
Popis: Babesia ovis is a tick‐transmitted protozoan haemoparasite causing ovine babesiosis in sheep and goats leading to considerable economic loss in Turkey and neighbouring countries. There are no vaccines available, therapeutic drugs leave toxic residues in meat and milk, and tick vector control entails environmental risks. A panel of eight mini‐ and micro‐satellite marker loci was developed and applied to study genetic diversity and substructuring of B. ovis from western, central and eastern Turkey. A high genetic diversity (He = 0.799) was found for the sample of overall B. ovis population (n = 107) analyzed. Principle component analysis (PCoA) revealed the existence of three parasite subpopulations: (a) a small subpopulation of isolates from Aydin, western Turkey; (b) a second cluster predominantly generated by isolates from western Turkey; and (c) a third cluster predominantly formed by isolates from central and eastern Turkey. Two B. ovis isolates from Israel included in the analysis clustered with isolates from central and eastern Turkey. This finding strongly suggests substructuring of a major Turkish population into western versus central–eastern subpopulations, while the additional smaller B. ovis population found in Aydin could have been introduced, more recently, to Turkey. STRUCTURE analysis suggests a limited exchange of parasite strains between the western and the central–eastern regions and vice versa, possibly due to limited trading of sheep. Importantly, evidence for recombinant genotypes was obtained in regionally interchanged parasite isolates. Important climatic differences between the western and the central/eastern region, with average yearly temperatures of 21°C versus 15°C, correspond with the identified geographical substructuring. We hypothesize that the different climatic conditions may result in variation in the activity of subpopulations of Rhipicephalus spp. tick vectors, which, in turn, could selectively maintain and transmit different parasite populations. These findings may have important implications for vaccine development and the spread of drug resistance. Instituto de Patobiología Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Unlu, Ahmet Hakan. Van Yuzuncu Yil University. Vocational School of Gevas; Turquía Fil: Bilgic, Huseyin Bilgin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Bakirci, Serkan. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Hacilarlioglu, Selin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Karagenc, Tulin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Weir, William. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido Fil: Shiels, Brian. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido Fil: Shkap, Varda. Kimron Veterinary Institute. Division of Parasitology; Israel Fil: Aktas, Munir. Firat University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Databáze: OpenAIRE
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