Mutation of Residue βF71 of Escherichia coli Penicillin Acylase Results in Enhanced Enantioselectivity and Improved Catalytic Properties
| Autor: | Irina V. Shapovalova, Dick B. Janssen, D.B. Švedas, E. de Vries, Wynand Alkema, O.V. Jamskova, D.T. Guranda |
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| Přispěvatelé: | Biotechnology, Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| Rok vydání: | 2009 |
| Předmět: |
penicillin acylase
medicine.drug_class Chemistry Stereochemistry Cephalosporin Mutant βF71 MUTANTS ABBREVIATIONS: PA – PENICILLIN ACYLASE NTN – N-TERMINAL NUCLEOPHILE WT – WILD TYPE Phenylalanine medicine.disease_cause Biochemistry Catalysis Residue (chemistry) Nucleophile beta F71 mutants enantioselectivity medicine Molecular Medicine improved catalysis Enantiomer Molecular Biology Escherichia coli Research Article Biotechnology |
| Zdroj: | Acta naturae, 1(3), 94-98 Acta Naturae |
| ISSN: | 2075-8251 |
| Popis: | Residue phenylalanine 71 of the beta-chain of penicillin acylase from E. coil is involved in substrate binding and chiral discrimination of its enantiomers. Different amino acid residues have been introduced at position beta F71, and the mutants were studied with respect to their enantioselectivity and substrate specificity. Some mutants demonstrated remarkably improved catalytic activity. Moreover, mutation of beta F71 residue allowed to enhance penicillin acylase enantioselectivity. The catalytic activity to the specific substrates was improved up to 36 times, most notably for K, R, and L mutants. Increased activity to a D-phenylglycine derivative - a valuable specificity improvement for biocatalytic synthesis of new penicillins and cephalosporins - was shown for beta F71R and beta F7IL mutants. The synthetic capacity of penicillin acylase with 6-aminopenicillanic acid as an external nucleophile was especially sensitive to mutation of the beta 71 residue in contrast to the synthesis with 7-aminodeacetoxycephalosporanic acid. |
| Databáze: | OpenAIRE |
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