A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction

Autor:	Dayong Li, Chengjie Duan, Wenting Cheng, Youjing gong, Yanheng Yao, Xiaoping Wang, Zhongyun Wang, Yang Xiang
Rok vydání:	2022
Předmět:	SARS-CoV-2 Materials Chemistry Metals and Alloys Ceramics and Composites COVID-19 Humans RNA Viral General Chemistry CRISPR-Cas Systems Nucleic Acid Amplification Techniques Catalysis Surfaces Coatings and Films Electronic Optical and Magnetic Materials
Zdroj:	Chemical Communications. 58:4484-4487
ISSN:	1364-548X 1359-7345
DOI:	10.1039/d2cc00488g
Popis:	A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation from the target sequence, demonstrating its superior analytical performance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03be4b17b22b590497c6ce73688b00c2 https://doi.org/10.1039/d2cc00488g Zobrazit plný text záznamu