Promoter methylation of DAPK1, FHIT, MGMT, and CDKN2A genes in cervical carcinoma
| Autor: | Jinhua Quan, Kosuke Yoshihara, Koji Nishino, Kenichi Tanaka, Tetsuro Yahata, Chiaki Banzai, Masayuki Sekine |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Uterine Cervical Neoplasms Cervical intraepithelial neoplasia medicine.disease_cause FHIT medicine Humans Promoter Regions Genetic neoplasms DNA Modification Methylases Cyclin-Dependent Kinase Inhibitor p16 Cervical cancer Intraepithelial neoplasia business.industry Tumor Suppressor Proteins Hematology General Medicine Methylation DNA Methylation Middle Aged medicine.disease Acid Anhydride Hydrolases Neoplasm Proteins Gene Expression Regulation Neoplastic Death-Associated Protein Kinases DNA Repair Enzymes Oncology DNA methylation Cancer research Carcinoma Squamous Cell Adenocarcinoma Surgery Female business Carcinogenesis Carcinoma in Situ |
| Zdroj: | International journal of clinical oncology. 19(1) |
| ISSN: | 1437-7772 |
| Popis: | Aberrant DNA methylation contributes to the malignant phenotype in virtually all types of human cancer. This study explored the relationship between promoter methylation and inactivation of the DAPK1, FHIT, MGMT, and CDKN2A genes in cervical cancer. The promoter methylation of DAPK1, FHIT, MGMT, and CDKN2A was investigated by using a methylation-specific polymerase chain reaction in 53 specimens of cervical cancer (42 squamous cell carcinoma, 11 adenocarcinoma), 22 specimens of intraepithelial neoplasia tissues, and 24 control normal cervical tissue specimens. The correlation of promoter methylation with the clinicopathological features of cervical cancer was analyzed. The expressions of DAPK1, FHIT, MGMT, and CDKN2A were detected by measuring relative mRNA levels. The promoter methylation of DAPK1, FHIT, MGMT, and CDKN2A in cervical cancer vs. intraepithelial neoplasia vs. normal cervical tissue was 75.5 vs. 31.8 vs. 4.2 % (p |
| Databáze: | OpenAIRE |
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