αV Integrin Expression and Localization in Male Germ Cells
| Autor: | Katerina Komrskova, Pavla Postlerova, Veronika Palenikova, Eliska Valaskova, Michaela Frolikova |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
pig endocrine system Spermiogenesis Swine QH301-705.5 Acrosome reaction Integrin sperm Catalysis Article Inorganic Chemistry Mice Capacitation medicine Animals Humans αV integrin human Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy mouse biology urogenital system male germ cells Acrosome Reaction Organic Chemistry General Medicine Integrin alphaV Sperm Spermatozoa Computer Science Applications Cell biology Chemistry medicine.anatomical_structure biology.protein Gamete Inner acrosomal membrane Spermatogenesis |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 9525, p 9525 (2021) International Journal of Molecular Sciences Volume 22 Issue 17 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Integrins are transmembrane receptors that facilitate cell adhesion and cell–extracellular matrix communication. They are involved in the sperm maturation including capacitation and gamete interaction, resulting in successful fertilization. αV integrin belongs to the integrin glycoprotein superfamily, and it is indispensable for physiological spermiogenesis and testosterone production. We targeted the gene and protein expression of the αV integrin subunit and described its membrane localization in sperm. Firstly, in mouse, we traced αV integrin gene expression during spermatogenesis in testicular fraction separated by elutriation, and we detected gene activity in spermatogonia, spermatocytes, and round spermatids. Secondly, we specified αV integrin membrane localization in acrosome-intact and acrosome-reacted sperm and compared its pattern between mouse, pig, and human. Using immunodetection and structured illumination microscopy (SIM), the αV integrin localization was confined to the plasma membrane covering the acrosomal cap area and also to the inner acrosomal membrane of acrosome-intact sperm of all selected species. During the acrosome reaction, which was induced on capacitated sperm, the αV integrin relocated and was detected over the whole sperm head. Knowledge of the integrin pattern in mature sperm prepares the ground for further investigation into the pathologies and related fertility issues in human medicine and veterinary science. |
| Databáze: | OpenAIRE |
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