Incorporation of 5-bromo-2'-deoxyuridine into DNA and proliferative behavior of cerebellar neuroblasts : all that glitters is not gold
| Autor: | Martí-Clúa, Joaquín, Universitat Autònoma de Barcelona. Institut de Neurociències |
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| Rok vydání: | 2021 |
| Předmět: |
Cerebellum
external granular layer QH301-705.5 Neurogenesis Cerebellar neuroepithelium Central nervous system Apoptosis Review Biology Cell cycle cerebellar neuroepithelium Pyrimidine analogue chemistry.chemical_compound Neuroblast Neural Stem Cells medicine External granular layer Animals Humans neurogenetic timetables Biology (General) Perinatal life Neurogenetic gradients neurogenetic gradients prenatal life DNA synthesis Staining and Labeling Prenatal life apoptosis General Medicine DNA Deoxyuridine Cell biology perinatal life medicine.anatomical_structure chemistry Bromodeoxyuridine 5-bromo-2′-deoxyuridine Neurogenetic timetables 5-bromo-2'-deoxyuridine cell cycle Nucleoside |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Cells, Vol 10, Iss 1453, p 1453 (2021) Cells |
| Popis: | The synthetic halogenated pyrimidine analog, 5-bromo-2′-deoxyuridine (BrdU), is a marker of DNA synthesis. This exogenous nucleoside has generated important insights into the cellular mechanisms of the central nervous system development in a variety of animals including insects, birds, and mammals. Despite this, the detrimental effects of the incorporation of BrdU into DNA on proliferation and viability of different types of cells has been frequently neglected. This review will summarize and present the effects of a pulse of BrdU, at doses ranging from 25 to 300 µg/g, or repeated injections. The latter, following the method of the progressively delayed labeling comprehensive procedure. The prenatal and perinatal development of the cerebellum are studied. These current data have implications for the interpretation of the results obtained by this marker as an index of the generation, migration, and settled pattern of neurons in the developing central nervous system. Caution should be exercised when interpreting the results obtained using BrdU. This is particularly important when high or repeated doses of this agent are injected. I hope that this review sheds light on the effects of this toxic maker. It may be used as a reference for toxicologists and neurobiologists given the broad use of 5-bromo-2′-deoxyuridine to label dividing cells. |
| Databáze: | OpenAIRE |
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