Evaluation of different biological and chemical treatments in agroindustrial residues for the production of fungal glucanases and xylanases
| Autor: | Tania Sila Campioni, Pedro de Oliva-Neto, Douglas Fernandes Silva, Ludmila Melo Hergesel, Ana Flávia Azevedo Carvalho |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Enzyme complex Bioengineering Cellulase 01 natural sciences Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences chemistry.chemical_compound Bioenergy 010608 biotechnology Bioreactor Stirred tank bioreactor Food science Cellulose Submerged fermentation (SmF) Trichoderma reesei Fibrolytic enzyme complex biology Chemistry technology industry and agriculture equipment and supplies biology.organism_classification Trichoderma reesei QM9414 030104 developmental biology Shake-flask cultures biology.protein Xylanase Bagasse |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1359-5113 |
| Popis: | Made available in DSpace on 2018-12-11T17:18:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The production and use of fibrolytic enzyme complex in the hydrolysis of lignocellulosic materials is an important strategy for renewable bioenergy. The different carbon sources (residues) with or without some pretreatments (biological or chemical) were analyzed in order to increase the production of fibrolytic enzyme. Glycosyl hydrolases and xylanases were produced using Trichoderma reesei QM9414. The influence of some crude or pre-treated agro-industrial residues as a carbon source was previously investigated using shake-flask cultures. Subsequently, the selection of the best culture medium was studied under different pH and temperature conditions in stirred tank bioreactor. Fibrolytic activities reached a maximum of 0.85 ± 0.07 FPU mL−1 (total cellulase), 3.14 ± 0.01 CMC mL−1 (endoglucanase) and 1.25 ± 0.14 U mL−1 (exoglucanase) with the orange peel residue; and 93.08 ± 3.27 U mL−1 (xylanase) with sugarcane bagasse under alkali pretreatment. In the stirred tank bioreactor the cellulolytic activity increased to 1.76 ± 0.00 FPU mL−1, about 2 times higher than in the shake-flasks or under studied conditions. The biosynthesis of the fibrolytic complex using agroindustrial residues supplemented was shown to reach a higher total cellulose production. Biotechnology Department São Paulo State University UNESP Campus Biochemistry and Microbiology Department UNESP Campus Biotechnology Department São Paulo State University UNESP Campus Biochemistry and Microbiology Department UNESP Campus |
| Databáze: | OpenAIRE |
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