Screening of High Temperature-Tolerant Oleaginous Diatoms
Autor: | Lingxiang Zhang, Fan Hu, Yufang Pan, Xiu Wan, Hanhua Hu |
---|---|
Rok vydání: | 2020 |
Předmět: |
Thermotolerance
0106 biological sciences China Hot Temperature Biomass Raw material 01 natural sciences Applied Microbiology and Biotechnology Fatty Acids Monounsaturated 010608 biotechnology RNA Ribosomal 18S Food science Triglycerides Diatoms Fistulifera Biodiesel biology Chemistry Fatty Acids Temperature General Medicine biology.organism_classification Cyclotella sp Lipids Diatom Biofuels Lipid content Nitzschia palea Seasons Biotechnology |
Zdroj: | Journal of Microbiology and Biotechnology. 30:1072-1081 |
ISSN: | 1738-8872 1017-7825 |
Popis: | Screening suitable strains with high temperature adaptability is of great importance for reducing the cost of temperature control in microalgae cultivation, especially in summer. To obtain high temperature-tolerant diatoms, water samples were collected in summer from 7 different regions of China across the Northeast, North and East. A total of 731 water samples was collected and from them 131 diatom strains were isolated and identified based on the 18S rRNA sequences. Forty-nine strains out of the 131 diatoms could survive at 30°C, and 6 strains with relatively high biomass and lipid content at high temperature were selected and were found to be able to grow at 35°C. Cyclotella sp. HB162 had the highest dry biomass of 0.46 g/l and relatively high triacylglycerol (TAG) content of 237.4 mg/g dry biomass. The highest TAG content of 246.4 mg/g dry biomass was obtained in Fistulifera sp. HB236, while Nitzschia palea HB170 had high dry biomass (0.33 g/l) but relatively low TAG content (105.9 mg/g dry biomass). N. palea HB170 and Fistulifera sp. HB236 presented relatively stable growth rates and lipid yields under fluctuating temperatures ranging from 28 to 35°C, while Cyclotella HB162 maintained high lipid yield at temperatures below 25°C. The percentage of saturated fatty acids and monounsaturated fatty acids in all the 6 strains was 84-91% in total lipids and 90-94% in TAGs, which makes them the ideal feedstock for biodiesel. |
Databáze: | OpenAIRE |
Externí odkaz: |