ELEquant: a developmental framework and validation of forensic and conservation real-time PCR assays
| Autor: | Courtney Mower, Shanan S. Tobe, Margret J Potoczniak, Jillian Conte |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Conservation of Natural Resources Elephants Endangered species Wildlife Biology Real-Time Polymerase Chain Reaction African elephant 03 medical and health sciences 0302 clinical medicine Species Specificity Asian elephant biology.animal Genetics Animals Molecular Biology Mouth Mucosa Reproducibility of Results Poaching General Medicine DNA Fingerprinting Data science Forensic science 030104 developmental biology Habitat destruction 030220 oncology & carcinogenesis Identification (biology) Microsatellite Repeats |
| Zdroj: | Molecular Biology Reports. 46:2093-2100 |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | A framework for the development and validation of a qPCR assay for species identification and DNA quantification for conservation and forensic purposes is presented. Elephants are commonly poached for their ivory tusks, which is the primary driving force behind their endangered status. In addition to poaching and trade, habitat loss due to logging and mining has also resulted in loss of elephants. Crimes against animals can be deterred and/or further prosecution sought through testing with forensic genetic techniques. The creation of novel genetic assays can greatly impact wildlife forensic science investigations in identifying the species. Molecular genetic techniques can help enforce conservation efforts; however, they must be properly developed and validated to be of evidentiary quality for court systems. African and Asian elephant buccal cells were used as model in this work. The assay provides a method to differentiate biological fluids of both genera of elephants simultaneously. It can be used for identification of elephant derived products and presents valuable quantification for optimized further testing, such as microsatellite detection. |
| Databáze: | OpenAIRE |
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