A Sericin-Derived Peptide Protects Sf9 Insect Cells from Death Caused by Acute Serum Deprivation
| Autor: | Hideyuki Yamada, Youichi Kato, Hiroshi Takagi, Shigeru Nakamori, Kazuhisa Tsujimoto, Masakazu Takahashi |
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| Rok vydání: | 2005 |
| Předmět: |
Programmed cell death
Time Factors Cell Survival Molecular Sequence Data Cell Fibroin Bioengineering Sf9 Peptide Spodoptera Biology Applied Microbiology and Biotechnology Sericin Culture Media Serum-Free Hydrolysate Cell Line medicine Animals Amino Acid Sequence Sericins Cell Proliferation chemistry.chemical_classification Dose-Response Relationship Drug fungi General Medicine Peptide Fragments Amino acid medicine.anatomical_structure chemistry Biochemistry Biotechnology |
| Zdroj: | Biotechnology Letters. 27:893-897 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-005-7177-5 |
| Popis: | Sericin is the silk protein enveloping fibroin fibers in cocoons. Sericin hydrolysate protects cultured Sf9 insect cells from death caused by serum deprivation; the activity depends on the repeats of 38 amino acids. A partial peptide from the 38 residues, SGGSSTYGYS, inhibited serum-deprivation death as well. Cell viabilities in the presence of 10% (v/v) foetal calf serum, no additives and 1 mM: SGGSSTYGYS were 96, 12 and 31% on the third day after inoculation, respectively. Aromatic residues seemed to be important because SGGSSTWGWS had the same activity as SGGSSTYGYS but SGGSSTAGAS had no activity. |
| Databáze: | OpenAIRE |
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