Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart
| Autor: | Junhui Sun, Fan Zhang, Christian A. Combs, Robert S. Balaban, Anna Kosmach, Barbara Roman, Armel N. Femnou, Chengyu Liu, Elizabeth Murphy |
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| Rok vydání: | 2021 |
| Předmět: |
spectroscopy
Time Factors QH301-705.5 Cell Energetics chemistry.chemical_element heart Mitochondrion Calcium General Biochemistry Genetics and Molecular Biology Germline Mitochondria Heart Mitochondrial Proteins Adrenergic stimulation Animals Humans Myocytes Cardiac Calcium Signaling Biology (General) Fluorescent Dyes Mice Knockout Chemistry Optical Imaging Isoproterenol Isolated Heart Preparation Metabolism Adrenergic beta-Agonists Myocardial Contraction Cell biology mitochondria Mice Inbred C57BL HEK293 Cells Calcium Channels Signal transduction Energy Metabolism Heterocyclic Compounds 3-Ring Intracellular |
| Zdroj: | Cell Reports, Vol 37, Iss 3, Pp 109846-(2021) |
| ISSN: | 2211-1247 |
| Popis: | Summary Optical methods for measuring intracellular ions including Ca2+ revolutionized our understanding of signal transduction. However, these methods are not extensively applied to intact organs due to issues including inner filter effects, motion, and available probes. Mitochondrial Ca2+ is postulated to regulate cell energetics and death pathways that are best studied in an intact organ. Here, we develop a method to optically measure mitochondrial Ca2+ and demonstrate its validity for mitochondrial Ca2+ and metabolism using hearts from wild-type mice and mice with germline knockout of the mitochondria calcium uniporter (MCU-KO). We previously reported that germline MCU-KO hearts do not show an impaired response to adrenergic stimulation. We find that these MCU-KO hearts do not take up Ca2+, consistent with no alternative Ca2+ uptake mechanisms in the absence of MCU. This approach can address the role of mitochondrial Ca2+ to the myriad of functions attributed to alterations in mitochondrial Ca2+. |
| Databáze: | OpenAIRE |
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