Preparation, characterization, and bioactivity evaluation of oligosaccharides from Atractylodes lancea (Thunb.) DC
| Autor: | Hui-yang Wang, Ying-yue Zhang, Dan Zhuang, Li-juan Meng, Chun-yao Liu, Guang-ping Lv |
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| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Polymers and Plastics Oligosaccharides Degree of polymerization Nitric Oxide chemistry.chemical_compound Ingredient Mice Liquid chromatography–mass spectrometry Materials Chemistry Animals Lymphocytes chemistry.chemical_classification Chromatography biology Hydrophilic interaction chromatography Macrophages Organic Chemistry Fast protein liquid chromatography Atractylodes Oligosaccharide biology.organism_classification Monomer RAW 264.7 Cells chemistry Atractylodes lancea |
| Zdroj: | Carbohydrate polymers. 277 |
| ISSN: | 1879-1344 |
| Popis: | Sixteen oligosaccharide monomers with the degree of polymerization 3 to 18 (DP 3 to DP 18) and three active fractions (DP 3-9, DP 8-11, and DP 11-17) were separated from Atractylodes lancea (Thunb.) DC. by optimized fast protein liquid chromatography coupled with refractive index detector (FPLC-RID) and preparation hydrophilic interaction chromatography (Pre-HILIC). Gas chromatography-mass spectrometer (GC–MS), liquid chromatography tandem mass spectrometry (LC-MS/MS), nuclear magnetic resonance (NMR) spectroscopy, and methylation analysis showed that the oligosaccharide in A. lancea was 1-kestose [β-D-fructofuranosyl-(2 → 1)-β-D-fructofuranosyl-(2 → 1)-α-D-glucopyranoside] (inulin-type fructooligosaccharides, FOS). Particularly, DP 3-9 showed the best capacity in stimulating phagocytic, NO, and cytokines production on RAW264.7 cells than any other purified oligosaccharide monomers and active fractions. It could also activate T-cells in Peyer's patch cells and enhance the production of colony stimulation factors. Besides, FPLC-RID showed a good capacity for large-scale preparation of DP 3-9 with the recovery of more than 93%. The bioactivity of sixteen FOS monomers (DP 3 to DP 18) and three FOS fractions (DP 3-9, DP 8-11, and DP 11-17) investigated in this study are beneficial for the utilization of FOS as a functional ingredient in novel product development. |
| Databáze: | OpenAIRE |
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