Single cell transcriptomes reveal expression patterns of chemoreceptor genes in olfactory sensory neurons of the Caribbean spiny lobster, Panulirus argus
Autor: | Mihika T. Kozma, Charles D. Derby, Matthew T. Rump, Hanh Ngo-Vu, Barry W. Ache, Yuriy V. Bobkov |
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Rok vydání: | 2020 |
Předmět: |
Chemoreceptor
lcsh:QH426-470 lcsh:Biotechnology Receptor expression TRPV Cation Channels Sensory system Olfaction Biology Receptors Ionotropic Glutamate Receptors G-Protein-Coupled Ionotropic receptor Olfactory sensory neuron Single cell transcriptome 03 medical and health sciences Transient receptor potential channel 0302 clinical medicine Crustacea lcsh:TP248.13-248.65 TRP channel Genetics Animals RNA-Seq Palinuridae Epithelial Sodium Channels Receptor 030304 developmental biology G protein-coupled receptor 0303 health sciences Spiny lobster Chemoreceptor Cells Cell biology lcsh:Genetics G-protein coupled receptor Single-Cell Analysis Signal transduction Transcriptome 030217 neurology & neurosurgery Research Article Biotechnology |
Zdroj: | BMC Genomics, Vol 21, Iss 1, Pp 1-19 (2020) BMC Genomics |
ISSN: | 1471-2164 |
Popis: | Background Crustaceans express several classes of receptor genes in their antennules, which house olfactory sensory neurons (OSNs) and non-olfactory chemosensory neurons. Transcriptomics studies reveal that candidate chemoreceptor proteins include variant Ionotropic Receptors (IRs) including both co-receptor IRs and tuning IRs, Transient Receptor Potential (TRP) channels, Gustatory Receptors, epithelial sodium channels, and class A G-protein coupled receptors (GPCRs). The Caribbean spiny lobster, Panulirus argus, expresses in its antennules nearly 600 IRs, 17 TRP channels, 1 Gustatory Receptor, 7 epithelial sodium channels, 81 GPCRs, 6 G proteins, and dozens of enzymes in signaling pathways. However, the specific combinatorial expression patterns of these proteins in single sensory neurons are not known for any crustacean, limiting our understanding of how their chemosensory systems encode chemical quality. Results The goal of this study was to use transcriptomics to describe expression patterns of chemoreceptor genes in OSNs of P. argus. We generated and analyzed transcriptomes from 7 single OSNs, some of which were shown to respond to a food odor, as well as an additional 7 multicell transcriptomes from preparations containing few (2–4), several (ca. 15), or many (ca. 400) OSNs. We found that each OSN expressed the same 2 co-receptor IRs (IR25a, IR93a) but not the other 2 antennular coIRs (IR8a, IR76b), 9–53 tuning IRs but only one to a few in high abundance, the same 5 TRP channels plus up to 5 additional TRPs, 12–17 GPCRs including the same 5 expressed in every single cell transcriptome, the same 3 G proteins plus others, many enzymes in the signaling pathways, but no Gustatory Receptors or epithelial sodium channels. The greatest difference in receptor expression among the OSNs was the identity of the tuning IRs. Conclusions Our results provide an initial view of the combinatorial expression patterns of receptor molecules in single OSNs in one species of decapod crustacean, including receptors directly involved in olfactory transduction and others likely involved in modulation. Our results also suggest differences in receptor expression in OSNs vs. other chemosensory neurons. |
Databáze: | OpenAIRE |
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