Cryoprotectant toxicity in Caenorhabditis elegans
| Autor: | Garrett J. Schumacher, Thomas E. Johnson, Patricia M. Tedesco |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Ethylene Glycol
Cryoprotectant Afatinib ved/biology.organism_classification_rank.species Mutant General Biochemistry Genetics and Molecular Biology Cryopreservation 03 medical and health sciences Cryoprotective Agents 0302 clinical medicine medicine Animals Dimethyl Sulfoxide Caenorhabditis elegans Caenorhabditis elegans Proteins Model organism 030219 obstetrics & reproductive medicine Formamides biology ved/biology 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine biology.organism_classification Vitrification 040201 dairy & animal science Cell biology Transplantation Oxidative Stress Toxicity General Agricultural and Biological Sciences Signal Transduction medicine.drug |
| Zdroj: | Cryobiology. 86:71-76 |
| ISSN: | 0011-2240 |
| Popis: | We have looked at the effects of the cryoprotectant M22 upon viability in the model organism C. elegans. M22 is a well-known vitrification solution which has been successfully used in the laboratory to preserve organs destined for transplantation. M22 reduces survival of C. elegans in a concentration-dependent manner. M22 at concentrations of 10% (v/v) or higher inhibits progeny production and development. A few mutants in the ILS (insulin-like signaling) pathway of C. elegans are more resistant to the toxic effect of M22 compared to wild-type worms. Afatinib, an anti-cancer drug, protects against M22 toxicity. Afatinib by itself does not increase longevity. |
| Databáze: | OpenAIRE |
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