Polyelectrolyte nanocomplexes based on chitosan derivatives for wound healing application

Autor: Vasile Ostafe, Olivier Jordan, Lee Ann Laurent-Applegate, Viorica Patrulea, Gerrit Borchard
Rok vydání: 2018
Předmět:
Polymers
Oligopeptides/administration & dosage/chemistry
Pharmaceutical Science
02 engineering and technology
030226 pharmacology & pharmacy
Cell Line
Chitosan
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Polymers/chemistry
Dynamic light scattering
Hyaluronic acid
Cell Adhesion
Humans
Cell Adhesion/drug effects
Chondroitin sulfate
Wound Healing/drug effects
Skin
Chitosan/administration & dosage/chemistry
ddc:615
Wound Healing
Hydrogels
General Medicine
Fibroblasts
Hydrogen-Ion Concentration
Hydrogels/chemistry
021001 nanoscience & nanotechnology
Polyelectrolytes
Polyelectrolyte
Fibroblasts/drug effects
Polyelectrolytes/administration & dosage/chemistry
chemistry
Skin/drug effects
Self-healing hydrogels
Biophysics
Nanocarriers
0210 nano-technology
Wound healing
Oligopeptides
Biotechnology
Zdroj: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 140 (2019) pp. 100-108
ISSN: 1873-3441
0939-6411
Popis: Wound healing, when compromised, may be guided by biological cues such as Arg-Gly-Asp (RGD), a peptide known to induce cell adhesion and migration, eventually combined with adapted nanocarriers. Three different formulations were prepared and investigated in vitro for topical application. All formulations were based on carboxylated and trimethylated chitosan (CMTMC) displaying RGD. The polyelectrolyte nanocomplexes were prepared by mixing two oppositely charged polymers of CMTMC and chondroitin sulfate at different polymer ratios and subsequently characterized by dynamic light scattering and scanning electron microscopy. Hydrogels and foams with a high concentration of RGD-functionalized chitosan (3%) and hyaluronic acid (1.5%) that formed gel-embedded nanocomplexes were developed. In vitro assays showed absence of toxicity, ability to promote proliferation over 7 days and promotion of migration of human dermal fibroblasts treated with any of our formulations. These formulations were shown to be suitable for easy topical application and have the potential to accelerate wound healing.
Databáze: OpenAIRE
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