Surfactant protein C: hormonal control of SP-C mRNA levels in vitro
| Autor: | Joanna Floros, D. W. Dynia, I. Gross, Katherine V. Nichols, H. Lu, S. V. Veletza |
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| Rok vydání: | 1992 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Transcription Genetic Physiology Proteolipids Molecular Sequence Data 8-Bromo Cyclic Adenosine Monophosphate Stimulation Biology Dexamethasone Fetus Organ Culture Techniques Physiology (medical) Internal medicine Gene expression medicine Animals Humans Northern blot RNA Messenger Cloning Molecular Lung Gene Library Cell Nucleus Messenger RNA Analysis of Variance Base Sequence Surfactant protein C Pulmonary Surfactants Cell Biology Adenosine Rats Kinetics Endocrinology Bucladesine Oligonucleotide Probes medicine.drug Hormone |
| Zdroj: | The American journal of physiology. 262(6 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | We have studied hormonal regulation of the surfactant protein C (SP-C) in fetal 18-dah rat lung explants. SP-C mRNA was detected in Northern blots with a specific rat SP-C cDNA probe and quantified by densitometry. Treatment of the explants with dexamethasone resulted in a dose-dependent increase of the SP-C mRNA level. Transcriptional assays have shown that the regulation of SP-C mRNA by dexamethasone involves a transcriptional step. Administration of the cAMP analogues, 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) or dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), produced a dose-dependent increase of SP-C mRNA levels, with maximum stimulation observed at 200 microM. The thyroid hormone T3 had no effect on SP-C mRNA levels, whether administered alone or in combination with dexamethasone. Variation in the effects of the above hormones on three surfactant protein mRNAs, SP-A, SP-B and SP-C, indicates that the hormonal regulation of the surfactant proteins is a complex process and that each gene is, in part, differentially regulated. |
| Databáze: | OpenAIRE |
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