Identification of a New Broadly Cross-reactive Epitope within Domain III of the Duck Tembusu Virus E Protein
| Autor: | Ming Liu, Ronghong Hua, Qingshan Zhang, Wulin Shaozhou, Jyung-Hurng Liu, Chenxi Li, Runze Meng, Yun Zhang, Xiaofei Bai |
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| Rok vydání: | 2016 |
| Předmět: |
Models
Molecular 0301 basic medicine Phage display medicine.drug_class viruses Mutagenesis (molecular biology technique) Monoclonal antibody Article Epitope Virus Flavivirus Infections 03 medical and health sciences Protein Domains Viral Envelope Proteins Peptide Library medicine Animals Binding site Poultry Diseases Binding Sites Multidisciplinary biology Flavivirus virus diseases biology.organism_classification Virology Ducks 030104 developmental biology Amino Acid Substitution Mutation Epitopes B-Lymphocyte Epitope Mapping |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep36288 |
| Popis: | In 2010, a pathogenic flavivirus termed duck Tembusu virus (DTMUV) caused widespread outbreak of egg-drop syndrome in domesticated ducks in China. Although the glycoprotein E of DTMUV is an important structural component of the virus, the B-cell epitopes of this protein remains uncharacterized. Using phage display and mutagenesis, we identified a minimal B-cell epitope, 374EXE/DPPFG380, that mediates binding to a nonneutralizing monoclonal antibody. DTMUV-positive duck serum reacted with the epitope, and amino acid substitutions revealed the specific amino acids that are essential for antibody binding. Dot-blot assays of various flavivirus-positive sera indicated that EXE/DPPFG is a cross-reactive epitope in most flaviviruses, including Zika, West Nile, Yellow fever, dengue, and Japanese encephalitis viruses. These findings indicate that the epitope sequence is conserved among many strains of mosquito-borne flavivirus. Protein structure modeling revealed that the epitope is located in domain III of the DTMUV E protein. Together, these results provide new insights on the broad cross-reactivity of a B-cell binding site of the E protein of flaviviruses, which can be exploited as a diagnostic or therapeutic target for identifying, studying, or treating DTMUV and other flavivirus infections. |
| Databáze: | OpenAIRE |
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