Phage particles harboring antibiotic resistance genes in fresh-cut vegetables and agricultural soil
Autor: | Lorena Rodríguez-Rubio, Maryury Brown-Jaque, Olatz Larrañaga, Pablo Quirós, Clara Gómez-Gómez, Anicet R. Blanch, Maite Muniesa |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Genes Viral 030106 microbiology Biology medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Transduction (genetics) Vegetables medicine Bacteriophages Escherichia coli Soil Microbiology lcsh:Environmental sciences General Environmental Science lcsh:GE1-350 Pathogenic bacteria Agriculture Drug Resistance Microbial biology.organism_classification chemistry Spinach Mobile genetic elements DNA Bacteria Antibiotic resistance genes |
Zdroj: | Environment International, Vol 115, Iss, Pp 133-141 (2018) |
ISSN: | 0160-4120 |
Popis: | Bacteriophages are ubiquitously distributed prokaryotic viruses that are more abundant than bacteria. As a consequence of their life cycle, phages can kidnap part of their host's genetic material, including antibiotic resistance genes (ARGs), which released phage particles transfer in a process called transduction. The spread of ARGs among pathogenic bacteria currently constitutes a serious global health problem. In this study, fresh vegetables (lettuce, spinach and cucumber), and cropland soil were screened by qPCR for ten ARGs (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, blaOXA-48, blaVIM, mecA, sul1, qnrA, qnrS and armA) in their viral DNA fraction. The presence of ARGs in the phage DNA was analyzed before and after propagation experiments in an Escherichia coli host strain to evaluate the ability of the phage particles to infect a host. ARGs were found in the phage DNA fraction of all matrices, although with heterogeneous values. ARG prevalence was significantly higher in lettuce and soil, and the most common overall were β-lactamases. After propagation experiments, an increase in ARG densities in phage particles was observed in samples of all four matrices, confirming that part of the isolated phage particles were infectious. This study reveals the abundance of free, replicative ARG-containing phage particles in vegetable matrices and cropland soil. The particles are proposed as vehicles for resistance transfer in these environments, where they can persist for a long time, with the possibility of generating new resistant bacterial strains. Ingestion of these mobile genetic elements may also favor the emergence of new resistances, a risk not previously considered. Keywords: Bacteriophages, Antibiotic resistance genes, Cropland, Lettuce, Cucumber, Spinach |
Databáze: | OpenAIRE |
Externí odkaz: |