Evaluation of antioxidant potential of Heliotropium bacciferum Forssk extract and wound healing activity of its topical formulation in rat
| Autor: | Elham Jafari, Marzieh Sajadi Bami, H. Fathalipour-Rayeni, Payam Khazaeli, Hamid Reza Rahimi, Mojtaba Shakibaie, Mahboubeh Adeli-Sardou, P.M. Shaghooei, Hamid Forootanfar, Mehrnaz Mehrabani, Mandana Ohadi, Mohsen Doostmohammadi |
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| Rok vydání: | 2022 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Pharmaceutical Science Heliotropium medicine.disease_cause Antioxidants Ointments Superoxide dismutase chemistry.chemical_compound medicine Animals Rats Wistar Pharmacology chemistry.chemical_classification Wound Healing Reactive oxygen species biology Traditional medicine Plant Extracts Superoxide Dismutase Chemistry Glutathione Rats Catalase biology.protein Wound healing Oxidative stress |
| Zdroj: | Annales Pharmaceutiques Françaises. 80:280-290 |
| ISSN: | 0003-4509 |
| DOI: | 10.1016/j.pharma.2021.09.005 |
| Popis: | Wound healing is a dynamic process that occurs in the tissue under the skin. During this process, oxidative stress biomarkers are excessively produced, which finally lead to inflammation and cellular damage. In this study, efforts have been made to evaluate the antioxidant effect and wound healing activity topical formulation containing Heliotropium bacciferum Forssk extract. The in vitro antioxidant properties were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities and the ferric reducing antioxidant power (FRAP) assay. The herbal ointments (2.5% w/w and 5% w/w) were prepared from the hydroalcoholic extract of H. bacciferum Forssk and administrated on the induced wounds in Wistar rats. The chromatic assay, percentage of wound contraction, and histopathological studies were used for evaluating the wound healing activity. For the evaluation of reactive oxygen species (ROS), catalase (CAT) activity, superoxide dismutase (SOD), and glutathione (GSH) levels were examined. The DPPH method showed tremendous radical scavenging activities at the corresponding concentrations with EC50 value of 80 μg/mL. Topical application of the ointment (5% w/w) showed the highest wound contraction in comparison to the positive control (treated with CICALFATE™) and the control group (treated with normal saline). Similarly, the histological study of the group treated with the extract ointment (5% w/w) showed full collagen tissue deposition with a complete epidermal regeneration. The results of the assessment of GSH levels as well as CAT and SOD activities in the treated group (5% w/w) confirmed the scavenging property of the extract ointment. Our findings indicated the proper wound healing impact of the topical formulation of H. bacciferum Forssk due to its notable antioxidant capacity. |
| Databáze: | OpenAIRE |
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