In VitroEvaluation of the Ophthalmic Toxicity Profile of Chlorhexidine and Propamidine Isethionate Eye Drops
| Autor: | Anxo Fernández-Ferreiro, José Blanco Méndez, María Teresa Rodríguez-Ares, Antonio Piñeiro-Ces, María Pardo, Victoria Díaz-Tomé, Maria Santiago-Varela, María Jesús Lamas, Miguel González-Barcia, Francisco J. Otero-Espinar, Andrea Luaces-Rodríguez, María Gil-Martínez |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Time Factors Cell Survival Chemistry Pharmaceutical medicine.medical_treatment Corneal Keratocytes Biology Animal Testing Alternatives Eye medicine.disease_cause Propamidine Keratitis Cornea 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ophthalmology medicine Animals Humans Pharmacology (medical) Viability assay Cells Cultured Pharmacology Dose-Response Relationship Drug Chlorhexidine Eye drop medicine.disease eye diseases Benzamidines Surgery 030104 developmental biology medicine.anatomical_structure chemistry Acanthamoeba keratitis 030221 ophthalmology & optometry Cattle Ophthalmic Solutions Irritation medicine.drug |
| Zdroj: | Journal of Ocular Pharmacology and Therapeutics. 33:202-209 |
| ISSN: | 1557-7732 1080-7683 |
| DOI: | 10.1089/jop.2016.0053 |
| Popis: | Purpose: Acanthamoeba keratitis causes frequent epithelial lesions that fully expose the corneal stroma. The aim of this study was to determine the toxic profile of chlorhexidine and propamidine eye drops. Methods: We used primary human keratocytes in cell culture in combination with a novel technology that evaluates dynamic real-time cytotoxicity through impedance analysis. Additional studies such as a classic cell viability test (WST-1®), a bovine corneal opacity and permeability assay, and an irritation eye study (Hen's Egg Test [HET]) have been made. Results: Both eye drop formulations showed a time- and concentration-dependent toxicity profile, in which long periods and high concentrations were more detrimental to cells. In prolonged times of exposure, propamidine is more harmful to cells than chlorhexidine. On the contrary, no irritation has been detected in using the HET-chorioallantoic membrane test and no alterations in the corneal transparency nor permeability was produced by the treatm... |
| Databáze: | OpenAIRE |
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