An Unusual Pattern of Spontaneous Mutations Recovered in the Halophilic Archaeon Haloferax volcanii
| Autor: | John W. Drake, Reena R. Mackwan, Geraldine T. Carver, Dennis W. Grogan |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Genetics
Mutation Cell division biology Orotate Phosphoribosyltransferase Haloferax volcanii Uracil medicine.disease_cause biology.organism_classification Genome Genes Archaeal chemistry.chemical_compound chemistry Notes medicine Direct repeat Orotate phosphoribosyltransferase Gene Repetitive Sequences Nucleic Acid |
| Zdroj: | Genetics. 176:697-702 |
| ISSN: | 1943-2631 |
| DOI: | 10.1534/genetics.106.069666 |
| Popis: | Spontaneous mutations in the orotate:phosphoribosyl transferase (pyrE2) gene of the halophilic archaeon Haloferax volcanii were selected by 5-fluoroorotic acid plus uracil at a rate of ∼2 × 10−8/cell division in fluctuation and null-fraction tests but ∼6 × 10−8/cell division in mutation-accumulation tests. The corresponding genomic mutation rates were substantially lower than those observed for other mesophilic microbial DNA genomes on the basis of similar target genes. The mutational spectrum was dominated by indels adding or deleting multiples of 3 bp. Properties of the organism contributing to this unusual mutational pattern may include phenotypic lag caused by a high chromosomal copy number and efficient promotion of strand misalignments by short direct repeats. |
| Databáze: | OpenAIRE |
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