Pregnancy-induced changes in metabolome and proteome in ovine uterine flushings†
| Autor: | Jessica E. Prenni, Jared J. Romero, Bethany E. Liebig, Thomas R. Hansen, Corey D. Broeckling |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteome Pregnancy Proteins Biology Endometrium Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy Metabolome medicine Animals Conceptus Estrous cycle Principal Component Analysis Sheep 030219 obstetrics & reproductive medicine Uterus Trophoblast Cell Biology General Medicine Interferon tau 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Reproductive Medicine Pregnancy Animal Female Corpus luteum |
| Zdroj: | Biology of Reproduction. 97:273-287 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Mass spectrometry (MS) approaches were used herein to identify metabolites and proteins in uterine flushings (UF) that may contribute to nourishing the conceptus. Ovine uteri collected on Day 12 of the estrous cycle (n = 5 ewes exposed to vasectomized ram) or Days 12 (n = 4), 14 (n = 5), or 16 (n = 5) of pregnancy (bred with fertile ram) were flushed using buffered saline. Metabolites were extracted using 80% methanol and profiled using ultraperformance liquid chromatography (LC) tandem mass spectrometry. The proteome was examined by digestion with trypsin, followed by the analysis of peptides with LC-MS/MS. Metabolite profiling detected 8510 molecular features of which 9 were detected only in UF from Day 14-16 pregnant ewes that function in fatty acid transport (carnitines), hormone synthesis (androstenedione like), and availability of nutrients (valine). Proteome analysis detected 783 proteins present by Days 14-16 of pregnancy in UF, 7 of which are as follows: annexin (ANX) A1, A2, and A5; calcium-binding protein (S100A11); profilin 1; trophoblast kunitz domain protein 1 (TKDP); and interferon tau (IFNT). These proteins function in endocytosis, exocytosis, calcium signaling, and inhibition of prostaglandins (annexins and S100A11); protecting against maternal proteases (TKDP); remodeling cytoskeleton (profilin 1); and altering uterine release of prostaglandin F2 alpha as well as inducing IFNT-stimulated genes in the endometrium and the corpus luteum (IFNT). Identifying metabolites and proteins produced by the uterus and conceptus advances our understanding of embryo/maternal signaling and provides insights into possible the causes of reproductive failure. |
| Databáze: | OpenAIRE |
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