Molecular characterization of the mouse tyrosinase gene: pigment cell-specific expression in transgenic mice
| Autor: | Erika Schmid, Ruth Ganss, Siegfried Ruppert, Günther Schütz, Friedrich Beermann |
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| Rok vydání: | 1992 |
| Předmět: |
Genetically modified mouse
Albinism Tyrosinase Transgene Clinical Biochemistry Gene Expression Iris Mice Transgenic Plant Science Biology Gene Expression Regulation Enzymologic Mice Gene expression medicine Animals Pigment Epithelium of Eye Promoter Regions Genetic Skin Genetics Regulation of gene expression Choroid Monophenol Monooxygenase Cell Biology Exons Hair follicle eye diseases Epithelium Cell biology medicine.anatomical_structure Phenotype Mutation Melanocytes sense organs Agronomy and Crop Science Developmental Biology Minigene Hair |
| Zdroj: | Pigment cell research. 5(5 Pt 2) |
| ISSN: | 0893-5785 |
| Popis: | Tyrosinase is the key enzyme in melanin synthesis, and is expressed in the pigment epithelium of the retina, a cell layer derived from the optic cup; and in neural crest-derived melanocytes of skin, hair follicle, choroid, and iris. The tyrosinase gene has been cloned and shown to map to the well-characterized c-locus (albino locus) of the mouse. Subsequent studies demonstrated that a functional tyrosinase minigene was able to rescue the albino phenotype in transgenic mice. The transgene was expressed in a cell type-specific manner in skin and eye. During development of the mouse, the tyrosinase gene is expressed in the pigment epithelium of the retina as early as day 10.5 of gestation. In the hair follicle, tyrosinase gene expression is detected from day 16.5 onwards. This cell-type-specific expression is largely reproduced in transgenic mice. Our results suggest that sequences in the immediate vicinity of the mouse tyrosinase gene are sufficient to provide cell type-specificity and developmental regulation in melanocytes and the pigment epithelium. |
| Databáze: | OpenAIRE |
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