Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis
| Autor: | Jean-Claude Raymond, Mohammad M. N. Esfahani, Chayakom Phurimsak, Pradip Patel, Patrick Broyer, Nicole Pamme, Maria Jose Lopez-Martinez, Bongkot Ngamsom |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Salmonella
Population 02 engineering and technology medicine.disease_cause 01 natural sciences Biochemistry Analytical Chemistry law.invention Dynabeads law medicine Environmental Chemistry Multiplex education Escherichia coli Spectroscopy education.field_of_study Chromatography Chemistry Immunomagnetic Separation 010401 analytical chemistry Sorting equipment and supplies 021001 nanoscience & nanotechnology 0104 chemical sciences Biotinylation Recombinant DNA Food Microbiology Microscopy Electron Scanning 0210 nano-technology |
| Zdroj: | Analytica chimica acta. 918 |
| ISSN: | 1873-4324 |
| Popis: | This study reports multiplex sorting of Salmonella typhimurium and Escherichia coli 0157, from broth cultures and from pathogen-spiked skinned chicken breast enrichment broths by employing microfluidic free-flow magnetophoresis. Magnetic beads of different sizes and magnetite content, namely Dynabeads anti-salmonella and Hyglos-Streptavidin beads together with the corresponding pathogen-specific biotinylated recombinant phages, were utilised as affinity solid phases for the capture and concentration of viable S. typhimurium and E. coli 0157. Following optimisation, the protocol was used to demonstrate continuous magnetophoretic sorting of the two pathogen-bound magnetic bead populations from mixed cultures and from pathogen-spiked chicken pre-enrichment broths under the influence of a Halbach magnet array. For example, in the latter case, a pure population of S. typhimurium-bound Dynabeads (72% recovery) was sorted from a 100 μL mixture containing E. coli 0157-bound Hyglos beads (67% recovery) within 1.2 min in the presence of 0.1% Tween 20. This proof-of-principle study demonstrates how more than one pathogen type can be simultaneously isolated/enriched from a single food pre-enrichment broth (e.g. Universal food enrichment broth). |
| Databáze: | OpenAIRE |
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