Anti-Candida albicans germ tube antibodies reduce in vitro growth and biofilm formation of C. albicans
| Autor: | Giulia Carrano, Simona Paulone, Elisabetta Blasi, María-Jesús Sevilla, María-Dolores Moragues, Lucía Lainz |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Colony-forming unit
0303 health sciences Antibodies Biofilm CAGTA Candida albicans Fungicidal activity Invasive candidiasis Microbiology Infectious Diseases biology 030306 microbiology Chemistry Germ tube Mycology Cell morphology biology.organism_classification In vitro Corpus albicans 03 medical and health sciences Antigen Biofilms Fungal Structures Antibodies Fungal |
| Popis: | Background Invasive candidiasis by Candida albicans is associated with high morbidity and mortality, due in part to the late implementation of an appropriate antifungal therapy hindered by the lack of an early diagnosis. Aims We aimed to evaluate the in vitro antifungal activity of the antibodies against C. albicans germ tubes (CAGTA) raised in a rabbit model of candidemia. Methods We measured the effect of CAGTA activity by colorimetric XTT and crystal violet assays, and colony forming units count, both on C. albicans planktonic cells and during the course of biofilm formation and maturation. Viability and cell morphology were assessed by optical, fluorescent or scanning electron microscopy. Results CAGTA ≥50 μg/ml caused a strong inhibition of C. albicans blastospores growth, and DiBAC fluorescent staining evidenced a fungicidal activity. Moreover, electron microscopy images revealed that CAGTA induced morphological alterations of the surface of C. albicans germ tubes grown free as well as in biofilm. Interestingly, CAGTA ≥80 μg/ml reduced the amount of C. albicans biofilm, and this effect started at the initial adhesion stage of the biofilm formation, during the first 90 min. Conclusions This is the first report showing that CAGTA reduce C. albicans growth, and impair its metabolic activity and ability to form biofilm in vitro. The antigens recognized by CAGTA could be the basis for the development of immunization protocols that might protect against Candida infections. |
| Databáze: | OpenAIRE |
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