Increasing the Assembly Efficacy of Peptidic β-Sheets for a Highly-Sensitive HIV Detection
| Autor: | Ye Liu, Yiming Shao, Xingyu Jiang, Zulan Li, Hongyan Sun, Yuan Gao, Xinli Deng, Yulong Cong, Hongyan Cao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Detection limit
Oligopeptide Chemistry Phenylalanine Human immunodeficiency virus (HIV) HIV Core Protein p24 HIV Congo Red Enzyme-Linked Immunosorbent Assay medicine.disease_cause Positive correlation Analytical Chemistry Highly sensitive Congo red chemistry.chemical_compound Blood Biochemistry Limit of Detection medicine Humans Protein Conformation beta-Strand Fluorescent Dyes Protein Binding |
| Zdroj: | Analytical chemistry. 92(16) |
| ISSN: | 1520-6882 |
| Popis: | Our recent publication illustrates the critical role of phenylalanine-mediated aromatic-aromatic interactions in determining the assembly of peptidic β-sheets. However, the effect of phenylalanine number on regulating the assembly efficacy of peptidic β-sheets remains poorly understood. We herein evaluate the assembly efficacy of β-sheets of a series of oligopeptides which contain 0, 1, 2, or 3 phenylalanine in their molecular backbones. In our assembly system, two phenylalanine (2F) is the minimum number for driving the assembly of β-sheets of oligopeptides. Oligopeptides with three phenylalanine (3F) show significantly increased assembly efficacy of β-sheets compared to that with 2F. These results suggest a positive correlation between the phenylalanine number and assembly efficacy of β-sheets. By improving the assembly efficacy of β-sheets, we further develop a highly sensitive HIV analytical system in which the specific binding of β-sheets with Congo Red induces enhanced fluorescence. For HIV p24 detection, the 3F-based analytical system (0.61 pg/mL) shows a significantly lower limit of detection (LOD) than the 2F-based analytical system (2.44 pg/mL), both of which are more sensitive than commercial ELISA (5 pg/mL) used in the clinic. This work not only illustrates the effect of phenylalanine number on regulating the assembly efficacy of β-sheets but also provides a guideline for the construction of a highly sensitive analytical system of disease diagnosis. |
| Databáze: | OpenAIRE |
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