Modulation of Ca2+-activated K+ channels in human vascular cells by insulin and basic fibroblast growth factor
| Autor: | Rainer Voisard, Johannes Waltenberger, Torsten Mattfeldt, Vinzenz Hombach, Andreas Hannekum, Katja Reineker, Johannes Wiecha, Michael Reitmayer |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Umbilical Veins
medicine.medical_specialty Patch-Clamp Techniques Potassium Channels Endothelium Arteriosclerosis Endocrinology Diabetes and Metabolism medicine.medical_treatment Basic fibroblast growth factor Scorpion Venoms Vasodilation Biology Muscle Smooth Vascular Umbilical vein Potassium Channels Calcium-Activated chemistry.chemical_compound Endocrinology Internal medicine medicine Humans Insulin Large-Conductance Calcium-Activated Potassium Channels Patch clamp Cells Cultured Membrane potential Coronary Vessels Potassium channel medicine.anatomical_structure chemistry Fibroblast Growth Factor 2 Endothelium Vascular Peptides |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1096-6374 |
| DOI: | 10.1016/s1096-6374(98)80108-1 |
| Popis: | Insulin and basic fibroblast growth factor (bFGF) play an important role in the pathogenesis of atherosclerosis and have been shown to have vasodilatory effects. Since modulation of vascular ion channels determines membrane potential and thereby influences essential Ca2+-dependent intracellular pathways, we have investigated the effect of insulin and bFGF on Ca2+-activated K+ channels (BKCa) in human umbilical vein endothelial cells (HUVEC) and smooth muscle cells. The latter were obtained from either atherosclerotic plaques (SMCP) or from media segments (SMCM) of human coronary arteries. Using the patch-clamp technique, insulin (100 microU/ml) caused a significant increase in BKCa open-state probability in SMCP and HUVEC, whereas no significant changes were observed in SMCM. Basic FGF (30 ng/ml) revealed a significant increase in BKCa activity in HUVEC and a significant decrease in the BKCa open-state probability in SMCP, but caused no changes in SMCM. Thus, growth factors modulate vascular BKCa in a cell-type specific manner, which may be of importance concerning vasoactive and atherogenic effects of growth factors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect