RNA Proximity Labeling: A New Detection Tool for RNA-Protein Interactions
| Autor: | Orit Hermesh, Saira Akram, Ralf-Peter Jansen, Lisa Heinold, Ronja Weissinger |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Immunoprecipitation
Aptamer RNA-binding protein Pharmaceutical Science Biotin Review RNA–protein complex Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Affinity chromatography lcsh:Organic chemistry Drug Discovery Gene expression Physical and Theoretical Chemistry ascorbate peroxidase 030304 developmental biology chemistry.chemical_classification 0303 health sciences DNA ligase Staining and Labeling Organic Chemistry RNA RNA-Binding Proteins Cell biology subcellular transcriptomics chemistry Chemistry (miscellaneous) Molecular Medicine Transcriptome biotin ligase 030217 neurology & neurosurgery proximity labeling Protein Binding |
| Zdroj: | Molecules, Vol 26, Iss 2270, p 2270 (2021) Molecules |
| ISSN: | 1420-3049 |
| Popis: | Multiple cellular functions are controlled by the interaction of RNAs and proteins. Together with the RNAs they control, RNA interacting proteins form RNA protein complexes, which are considered to serve as the true regulatory units for post-transcriptional gene expression. To understand how RNAs are modified, transported, and regulated therefore requires specific knowledge of their interaction partners. To this end, multiple techniques have been developed to characterize the interaction between RNAs and proteins. In this review, we briefly summarize the common methods to study RNA–protein interaction including crosslinking and immunoprecipitation (CLIP), and aptamer- or antisense oligonucleotide-based RNA affinity purification. Following this, we focus on in vivo proximity labeling to study RNA–protein interactions. In proximity labeling, a labeling enzyme like ascorbate peroxidase or biotin ligase is targeted to specific RNAs, RNA-binding proteins, or even cellular compartments and uses biotin to label the proteins and RNAs in its vicinity. The tagged molecules are then enriched and analyzed by mass spectrometry or RNA-Seq. We highlight the latest studies that exemplify the strength of this approach for the characterization of RNA protein complexes and distribution of RNAs in vivo. |
| Databáze: | OpenAIRE |
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