The effect of thymine starvation on chromosomal structure of escherichia coli JG-151
| Autor: | George E. Schaiberger, David L. Reichenbach, Bennett Sallman |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
DNA
Bacterial Starvation Sucrose Biophysics Cell Biology Chromosomes Bacterial Sedimentation Biology medicine.disease_cause Biochemistry Thymine chemistry.chemical_compound chemistry Chromosomal Structure Centrifugation Density Gradient Escherichia coli medicine medicine.symptom Molecular Biology Cell Division DNA Single strand |
| Zdroj: | Biochemical and Biophysical Research Communications. 42:23-30 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(71)90356-1 |
| Popis: | Labelled DNA extracted from control and thymine starved cells was qualitatively characterized with respect to sedimentation properties in alkaline sucrose gradients. DNA isolated from cells undergoing loss of division ability demonstrated decreasing sedimentation velocity. Sedimentation profiles of DNA extracted from cells which were starved for thymine under conditions which allowed spontaneous recovery of division ability to occur, demonstrated an increase in DNA sedimentation velocity toward normal control value. It appears that while thymine starvation can result in single strand breaks, this damage is not irreversible, for under certain conditions rejoining of the breaks can occur. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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