Inhibitory Effect of Vitamin C in Combination With Vitamin K3 on Tumor Growth and Metastasis of Lewis Lung Carcinoma Xenografted in C57BL/6 Mice

Autor:	Ming-Feng Chen, Cheng-Ming Su, Jiunn-Wang Liao, Chih-Min Yang, Miao-Lin Hu
Rok vydání:	2011
Předmět:	Male C57BL/6 Cancer Research medicine.medical_specialty Lung Neoplasms Blotting Western Transplantation Heterologous Medicine (miscellaneous) Ascorbic Acid Cell Line Carcinoma Lewis Lung Mice In vivo Cell Line Tumor Internal medicine Plasminogen Activator Inhibitor 1 medicine Animals Neoplasm Invasiveness Cell Proliferation Tissue Inhibitor of Metalloproteinase-2 Tissue Inhibitor of Metalloproteinase-1 Nutrition and Dietetics Dose-Response Relationship Drug biology Vitamin C Chemistry Vitamin K 3 Lewis lung carcinoma biology.organism_classification Ascorbic acid Urokinase-Type Plasminogen Activator Mice Inbred C57BL Transplantation Dose–response relationship Endocrinology Matrix Metalloproteinase 9 Oncology Matrix Metalloproteinase 2 Cisplatin Plasminogen activator
Zdroj:	Nutrition and Cancer. 63:1036-1043
ISSN:	1532-7914 0163-5581
DOI:	10.1080/01635581.2011.597537
Popis:	Vitamin C in combination with vitamin K3 (vit CK3) has been shown to inhibit tumor growth and lung metastasis in vivo, but the mechanism of action is poorly understood. Herein, C57BL/6 mice were implanted (s.c.) with Lewis lung carcinoma (LLC) for 9 days before injection (i.p.) with low-dose (100 mg vit C/kg + 1 mg vit K3/kg), high-dose (1,000 mg vit C/kg + 10 mg vit K3/kg) vit CK3 twice a week for an additional 28 days. As expected, vit CK3 or cisplatin (6 mg/kg, as a positive control) significantly and dose-dependently inhibited tumor growth and lung metastasis in LLC-bearing mice. Vit CK3 restored the body weight of tumor-bearing mice to the level of tumor-free mice. Vit CK3 significantly decreased activities of plasma metalloproteinase (MMP)-2, -9, and urokinase plasminogen activator (uPA). In lung tissues, vit CK3 1) increased protein expression of tissue inhibitor of metalloproteinase-1 (TIMP-1), TIMP-2, nonmetastatic protein 23 homolog 1 and plasminogen activator inhibitor-1; 2) reduced protein expression of MMP-2 and MMP-9; and 3) inhibited the proliferating cell nuclear antigen (PCNA). These results demonstrate that vit CK3 inhibits primary tumor growth and exhibits antimetastastic potential in vivo through attenuated tumor invasion and proliferation.
Databáze:	OpenAIRE
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