Use of High-Rate Ventilation Results in Enhanced Recellularization of Bioengineered Lung Scaffolds
| Autor: | Christer Sinderby, Gregory Phillips, Daisuike Taniguchi, Thomas K. Waddell, Pascal Duchesneau, Mohammadali Ahmadipour, Fabio Aoki, Golnaz Karoubi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cell
Biomedical Engineering Medicine (miscellaneous) Bioengineering Mice medicine Animals Pcr analysis Lung Tidal volume High rate Tissue Engineering Tissue Scaffolds business.industry Epithelial Cells respiratory system respiratory tract diseases Transplantation Perfusion medicine.anatomical_structure Breathing business Pulmonary Ventilation Biomedical engineering |
| Zdroj: | Tissue engineering. Part C, Methods. 27(12) |
| ISSN: | 1937-3392 |
| Popis: | While transplantation is a viable treatment option for end-stage lung diseases, this option is highly constrained by the availability of organs and postoperative complications. A potential solution is the use of bioengineered lungs generated from repopulated acellular scaffolds. Effective recellularization, however, remains a challenge. In this proof-of-concept study, mice lung scaffolds were decellurized and recellurized using human bronchial epithelial cells (BEAS2B). We present a novel liquid ventilation protocol enabling control over tidal volume and high rates of ventilation. The use of a physiological tidal volume (300 μL) for mice and a higher ventilation rate (40 breaths per minute vs. 1 breath per minute) resulted in higher cell numbers and enhanced cell surface coverage in mouse lung scaffolds as determined via histological evaluation, genomic polymerase chain reaction (PCR) analysis, and immunohistochemistry. A biomimetic lung bioreactor system was designed to include the new ventilation protocol and allow for simultaneous vascular perfusion. We compared the lungs cultured in our dual system to lungs cultured with a bioreactor allowing vascular perfusion only and showed that our system significantly enhances cell numbers and surface coverage. In summary, our results demonstrate the importance of the physical environment and forces for lung recellularization. Impact statement New bioreactor systems are required to further enhance the regeneration process of bioengineered lungs. This proof-of-concept study describes a novel ventilation protocol that allows for control over ventilation parameters such as rate and tidal volume. Our data show that a higher rate of ventilation is correlated with higher cell numbers and increased surface coverage. We designed a new biomimetic bioreactor system that allows for ventilation and simultaneous perfusion. Compared to a traditional perfusion only system, recellularization was enhanced in lungs recellularized with our new biomimetic bioreactor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|