Silver-nanoparticles increase bactericidal activity and radical oxygen responses against bacterial pathogens in human osteoclasts
| Autor: | Luis Filgueira, Nils Lannes, Michael Walch, Valerie Aurore, Marianne Blanchard, Solange Kharoubi Hess, Pierre-Yves Mantel, Fabienne Caldana |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Silver Biomedical Engineering Metal Nanoparticles Osteoclasts Pharmaceutical Science Medicine (miscellaneous) Virulence Bioengineering 02 engineering and technology Biology medicine.disease_cause Microbiology Bone Infection 03 medical and health sciences Phagocytosis medicine Humans Macrophage General Materials Science Cells Cultured chemistry.chemical_classification Reactive oxygen species Monocyte Staphylococcal Infections 021001 nanoscience & nanotechnology biology.organism_classification Anti-Bacterial Agents 030104 developmental biology medicine.anatomical_structure chemistry Immunology Molecular Medicine Reactive Oxygen Species 0210 nano-technology Intracellular Bacteria |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 14:601-607 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2017.11.006 |
| Popis: | Bone infections are difficult to treat and can lead to severe tissue destruction. Acute bone infections are usually caused by Staphylococcus aureus. Osteoclasts, which belong to the monocyte/macrophage lineage, are the key cells in bone infections. They are not well equipped for killing bacteria and may serve as a reservoir for bacterial pathogens. Silver has been known for centuries for its bactericidal activity. Here, we investigated the bactericidal effects of nano-silver particles in bacteria infected human osteoclasts. We found that nano-silver in per se non-toxic concentration enhanced the bactericidal activity in osteoclasts against intracellular Methicillin-resistant, virulent Staphylococcus aureus. The reduced bacterial survival in nano-silver pretreated cells correlated with increased reactive oxygen responses towards the invading pathogens. Overall, these results indicate that nano-silver compounds should be considered as an effective treatment and prevention option for bacterial bone and orthopedic implant infections. |
| Databáze: | OpenAIRE |
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