Population genomics diversity of Plasmodium falciparum in malaria patients attending Okelele Health Centre, Okelele, Ilorin, Kwara State, Nigeria
| Autor: | Olugbenga A. Mokuolu, Olatunji Matthew Kolawole, Tolulope Ololade Oloyede, Yetunde A. Olukosi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Plasmodium falciparum Merozoite Surface Protein genetic diversity medicine.medical_specialty Genotype 030231 tropical medicine Population Plasmodium falciparum Nigeria Biology Polymerase Chain Reaction Population genomics 03 medical and health sciences 0302 clinical medicine parasitic diseases medicine Humans Allele Merozoite surface protein education Alleles Genetic diversity education.field_of_study Public health Genetic Variation General Medicine Sequence Analysis DNA Articles medicine.disease biology.organism_classification Virology Malaria 030104 developmental biology Metagenomics human activities |
| Zdroj: | African Health Sciences; Vol 16, No 3 (2016); 704-711 |
| ISSN: | 1729-0503 1680-6905 |
| Popis: | Background : Plasmodium falciparum, the most dangerous malaria parasite species to humans remains an important public health concern in Okelele, a rural community in Ilorin, Kwara State, Nigeria. There is however little information about the genetic diversity of Plasmodium falciparum in Nigeria. Objective : To determine the population genomic diversity of Plasmodium falciparum in malaria patients attending Okelele Community Healthcare Centre, Okelele, Ilorin, Kwara State. Methods : In this study, 50 Plasmodium falciparum strains Merozoite Surface Protein 1, Merozoite Surface Protein 2 and Glutamate Rich Protein were analysed from Okelele Health Centre, Okelele, Ilorin, Nigeria. Genetic diversity of P. falciparum isolates were analysed from nested polymerase chain reactions (PCR) of the MSP-1 (K1, MAD 20 and RO33), MSP-2 (FC27 and 3D7) and Glutamate Rich Protein allelic families respectively. Results : Polyclonal infections were more in majority of the patients for MSP-1 allelic families while monoclonal infections were more for MSP-2 allelic families. Multiplicity of infection for MSP-1, MSP-2 and GLURP were 1.7, 1.8 and 2.05 respectively Conclusion : There is high genetic diversity in MSP – 2 and GLURP allelic families of Plasmodium falciparum isolates from Okelele Health Centre, Ilorin, Nigeria. Keywords : Plasmodium falciparum, Merozoite Surface Protein, genetic diversity. |
| Databáze: | OpenAIRE |
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