Magnetic Cross-Linked Enzyme Aggregates (mCLEAs) of Candida antarctica Lipase: An Efficient and Stable Biocatalyst for Biodiesel Synthesis
| Autor: | Juan L. Serra, María J. Llama, Carmen López, Álvaro Cruz-Izquierdo, Enrique A. Picó |
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| Rok vydání: | 2014 |
| Předmět: |
Time Factors
BIOCHEMISTRY AND MOLECULAR BIOLOGY Hydrolases lcsh:Medicine potential applications Biochemistry Enzyme Stability Biological Systems Engineering Chemical Precipitation Organic chemistry Bioenergy covalent immobilization Magnetite Nanoparticles lcsh:Science Fungal protein Biodiesel Multidisciplinary biology CLEAs Chemistry Esterases enantioselective hydrolysis Enzymes stabilization Cross-Linking Reagents AGRICULTURAL AND BIOLOGICAL SCIENCES Biofuel Physical Sciences Engineering and Technology iron-oxide nanoparticles performance Biotechnology Research Article Materials Science Biophysics Jatropha Bioengineering Biosynthesis Magnetic Materials Catalysis Fungal Proteins Protein Aggregates FE3O4 nanoparticles Lipase Molecular Biology Techniques Molecular Biology Materials by Attribute MEDICINE lcsh:R Biology and Life Sciences Transesterification Enzymes Immobilized equipment and supplies biology.organism_classification Energy and Power immobilized lipase Glutaral Biofuels Bionanotechnology Biocatalysis Enzymology Enzyme Immobilization biology.protein lcsh:Q Candida antarctica catalyzed transesterification |
| Zdroj: | PLoS ONE, Vol 9, Iss 12, p e115202 (2014) Addi. Archivo Digital para la Docencia y la Investigación instname PLoS ONE |
| ISSN: | 1932-6203 2011-2505 |
| Popis: | Enzyme-catalyzed production of biodiesel is the object of extensive research due to the global shortage of fossil fuels and increased environmental concerns. Herein we report the preparation and main characteristics of a novel biocatalyst consisting of Cross-Linked Enzyme Aggregates (CLEAs) of Candida antarctica lipase B (CALB) which are covalently bound to magnetic nanoparticles, and tackle its use for the synthesis of biodiesel from non-edible vegetable and waste frying oils. For this purpose, insolubilized CALB was covalently cross-linked to magnetic nanoparticles of magnetite which the surface was functionalized with –NH2 groups. The resulting biocatalyst combines the relevant catalytic properties of CLEAs (as great stability and feasibility for their reutilization) and the magnetic character, and thus the final product (mCLEAs) are superparamagnetic particles of a robust catalyst which is more stable than the free enzyme, easily recoverable from the reaction medium and reusable for new catalytic cycles. We have studied the main properties of this biocatalyst and we have assessed its utility to catalyze transesterification reactions to obtain biodiesel from non-edible vegetable oils including unrefined soybean, jatropha and cameline, as well as waste frying oil. Using 1% mCLEAs (w/w of oil) conversions near 80% were routinely obtained at 30°C after 24 h of reaction, this value rising to 92% after 72 h. Moreover, the magnetic biocatalyst can be easily recovered from the reaction mixture and reused for at least ten consecutive cycles of 24 h without apparent loss of activity. The obtained results suggest that mCLEAs prepared from CALB can become a powerful biocatalyst for application at industrial scale with better performance than those currently available. This work was supported by grants from the University of the Basque Country (UPV/EHU, project GIU11/25 and scholarship for AC-I, http://www.ehu.es), the Spanish Ministry of Economy and Competitiveness (project CTQ2011-25052, http://www.mineco.es), the Basque Government (project SAIOTEK S-PE12UN041, http://www.euskadi.net), Ikerbasque, the Basque Foundation for Science (fellowship for CL, www.ikerbasque.net) and European Union (project Energreen, POCTEFA EFA217/11, http://www.poctefa.eu/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Databáze: | OpenAIRE |
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