Chronic myelogenous leukemia-derived basophilic granulocytes express a functional active receptor for the anaphylatoxin C3a
| Autor: | Dieter Bitter-Suermann, Claudia Gietz, Andreas Klos, Titus Kretzschmar, Andreas Jeromin, Gerhard Rechkemmer, Jörg Köhl, Wilfried Bautsch |
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| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Anaphylatoxins Immunology chemical and pharmacologic phenomena Complement C5a Basophil Biology Pertussis toxin Binding Competitive Histamine Release chemistry.chemical_compound Internal medicine Leukemia Myelogenous Chronic BCR-ABL Positive medicine Immunology and Allergy Humans Anaphylatoxin Receptor Molecular biology Recombinant Proteins Basophils Receptors Complement Basophilic medicine.anatomical_structure Endocrinology chemistry biology.protein Complement C3a Calcium Interleukin-3 C3a receptor Signal transduction Histamine Signal Transduction |
| Zdroj: | European journal of immunology. 23(2) |
| ISSN: | 0014-2980 |
| Popis: | The receptor for the inflammatory peptide C3a has scarcely been examined on human cells. This work demonstrates that human tumor-derived basophilic granulocytes express C3a receptors, and presents parts of the hitherto unknown C3a-signal transduction. When incubated with IL-3, these cells specifically liberated histamine on C3a stimulation. Independent from IL-3, 240,000 +/- 100,000 receptors per cell with a Kd of 5.6 +/- 0.9 nM were determined. [Ca2+]i increased from 120 +/- 35 nM to 300 +/- 80 nM after a C3a challenge, as measured by digital imaging fluorescence microscopy, and rested at its basal level in the presence of C3a-desArg, the immediate catabolic product of C3a in vivo. This [Ca2+]i increase could be completely desensitized homologously by C3a as well as inhibited by up to 75% by pertussis toxin. Thus, tumor-derived basophils are suitable for cloning of the human C3a receptor. |
| Databáze: | OpenAIRE |
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